2 GROUPS OF RHINOVIRUSES REVEALED BY A PANEL OF ANTIVIRAL COMPOUNDS PRESENT SEQUENCE DIVERGENCE AND DIFFERENTIAL PATHOGENICITY
2 GROUPS OF RHINOVIRUSES REVEALED BY A PANEL OF ANTIVIRAL COMPOUNDS PRESENT SEQUENCE DIVERGENCE AND DIFFERENTIAL PATHOGENICITY
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DOI：
10.1128/jvi.64.3.1117-1123.1990
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发表时间：
1990-03-01
影响因子：
5.4
通讯作者：
JANSSEN, PAJ
中科院分区：
文献类型：
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作者：
ANDRIES, K;DEWINDT, B;JANSSEN, PAJ
多种化学性质不同的化合物可抑制几种鼻病毒血清型（普通感冒病毒）的复制。我们注意到，其中一种抗病毒化合物 WIN 51711 的抗病毒谱明显不同于共有谱或其他衣壳结合化合物，尽管它们都显示出共享相同的结合位点。因此，开始对所有已知的鼻病毒衣壳结合化合物针对所有血清型鼻病毒的系统评估。 结果的多变量分析揭示了两组鼻病毒的存在，我们将其称为抗病毒组 A 和 B。这些组的成员对抗病毒化合物的不同敏感性表明存在二态性结合位点。事实证明，抗病毒组在更广泛的层面上反映了鼻病毒血清型的差异。抗病毒谱的相似性与序列相似性高度相关，不仅与抗病毒化合物结合位点内的氨基酸相似，而且与整个 VP1 蛋白的氨基酸相似。此外，流行病学数据分析表明，B 组鼻病毒每种血清型产生的临床感染数是 A 组鼻病毒的两倍多。属于次要受体组的鼻病毒无一例外地都位于抗病毒 B 组的同一区域。
A variety of chemically different compounds inhibit the replication of several serotypes of rhinoviruses (common-cold viruses). We noticed that one of these antiviral compounds, WIN 51711, had an antiviral spectrum clearly distinctive from a consensus spectrum or other capsid-binding compound, although all of them were shown to share the same binding site. A systematic evaluation of all known rhinovirus capsid-binding compounds against all serotyped rhinoviruses was therefore initiated. Multivariate analysis of the results revealed the existence of two groups of rhinoviruses, which we will call antiviral groups A and B. The differential sensitivity of members of these groups to antiviral compounds suggests the existence of a dimorphic binding site. The antiviral groups turned out to be a reflection of a divergence of rhinovirus serotypes on a much broader level. Similarities in antiviral spectra were highly correlated with sequence similarities, not only of amino acids lining the antiviral compound-binding-site, but also of amino acids of the whole VP1 protein. Furthermore, analysis of epidemiological data indicated that group B rhinoviruses produced more than twice as many clinical infections per serotype than group A rhinoviruses did. Rhinoviruses belonging to the minor receptor groups were without exception all computed to lie in the same region of antiviral group B.