A NEW DIPLOID NONTUMORIGENIC HUMAN-BREAST EPITHELIAL-CELL LINE ISOLATED AND PROPAGATED IN CHEMICALLY DEFINED MEDIUM
A NEW DIPLOID NONTUMORIGENIC HUMAN-BREAST EPITHELIAL-CELL LINE ISOLATED AND PROPAGATED IN CHEMICALLY DEFINED MEDIUM
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DOI：
10.1007/bf02623578
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发表时间：
1987-03-01
期刊：
影响因子：
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通讯作者：
VANDEURS, B
中科院分区：
文献类型：
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作者：
BRIAND, P;PETERSEN, OW;VANDEURS, B
一种新的非致瘤性人乳腺上皮细胞系 HMT-3522 是从纤维囊性乳腺组织中建立的。将细胞在化学成分确定的培养基中进行移植和增殖，所述培养基包括胰岛素、转铁蛋白、表皮生长因子、氢化可的松、雌二醇、催乳素和亚硒酸钠。通过细胞角蛋白的免疫细胞化学检测确定细胞系的上皮性质。此外，电子显微镜显示单层极化细胞通过桥粒连接并具有顶端微绒毛。乳脂肪球膜抗原是正常管腔乳腺上皮细胞顶膜结构域的特异性，仅在汇合培养物中表达，其中一些细胞覆盖其他细胞，表明“干细胞”样特性。传代 25 至 30 次后，细胞成为二倍体，带有一些标记染色体，并且 D 组染色体丢失。这些细胞在无胸腺小鼠中不致瘤；它们缺乏雌激素受体，雌二醇不会刺激生长。 HMT-3522细胞系可能代表了体外乳腺细胞分化和癌变研究的有用模型。
A new, nontumorigenic human breast epithelial cell line, HMT-3522, has been established from fibrocystic breast tissue. Cells were explanted and propagated in chemically defined medium including insulin, transferrin, epidermal growth factor, hydrocortisone, estradiol, prolactin, and Na-selenite. The epithelial nature of the cell line was established by immunocytochemical detection of cytokeratins. Moreover, electronmicroscopy revealed monolayers of polarized cells connected by desmosomes and provided with apical microvilli. Milk fat globule membrene antigen, specific for the apical membrane domain of normal, luminal breast epithelial cells, was expressed only in confluent cultures where some cells overlaid others, indicating “stem cell”-like properties. After 25 to 30 passages, the cells are diploid with a few marker chromosomes and loss of chromosomes in the D-group. The cells are nontumorigenic in athymic mice; they lack estrogen receptors, and estradiol does not stimulate growth. The HMT-3522 cell line may represent a useful model for the study of brest cell differentiation and carcinogenesis in vitro.