Identification of Genes Relevant to Pesticides and Biology from Global Transcriptome Data of Monochamus alternatus Hope (Coleoptera: Cerambycidae) Larvae.
Identification of Genes Relevant to Pesticides and Biology from Global Transcriptome Data of Monochamus alternatus Hope (Coleoptera: Cerambycidae) Larvae.
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从松墨天牛（鞘翅目：天牛科）幼虫的整体转录组数据中鉴定与农药和生物学相关的基因。
DOI：
10.1371/journal.pone.0147855
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Zhang F
中科院分区：
文献类型：
--
作者：
Wu S;Zhu X;Liu Z;Shao E;Rebeca CL;Guo Y;Xiong Y;Mou Y;Xu R;Hu X;Liang G;Zou S;Guan X;Zhang F
松材线虫（Bursaphelenchus xylophilus）是中国松材线虫（Bursaphelenchus xylophilus）的主要病媒。虽然化学防治是预防松材萎蔫病的传统方法，但以生物防治为基础的新防治策略是防治松材萎蔫病的有效途径。然而，目前还没有描述该转录组的深序列分析，也没有关于该昆虫载体基因功能的信息。利用下一代测序技术对黄颡鱼4龄幼虫转录组进行全序列测序，成功构建黄颡鱼转录组数据库。总共有105,612个unique被分配到基因本体（GO）术语中，16,730个分类unique在COGs数据库中获得信息，13,024个unique与京都基因与基因组百科全书（KEGG）中的224条预测路径匹配。此外，还描述了与杀虫剂抗性相关的基因、RNAi、Bt受体、肠道消化酶、可能的未来昆虫控制靶点和免疫相关分子相关的基因。本研究提供了有价值的基础信息，可作为开发新的分子工具来制定褐毛霉防治策略的门户。
Monochamus alternatus Hope is the main vector in China of the Pine Wilt Disease caused by the pine wood nematode Bursaphelenchus xylophilus. Although chemical control is traditionally used to prevent pine wilt disease, new strategies based in biological control are promising ways for the management of the disease. However, there is no deep sequence analysis of Monochamus alternatus Hope that describes the transcriptome and no information is available about gene function of this insect vector. We used next generation sequencing technology to sequence the whole fourth instar larva transcriptome of Monochamus alternatus Hope and successfully built a Monochamus alternatus Hope transcriptome database. In total, 105,612 unigenes were assigned for Gene Ontology (GO) terms, information for 16,730 classified unigenes was obtained in the Clusters of Orthologous Groups (COGs) database, and 13,024 unigenes matched with 224 predicted pathways in the Kyoto Encyclopedia of Genes and Genome (KEGG). In addition, genes related to putative insecticide resistance-related genes, RNAi, the Bt receptor, intestinal digestive enzymes, possible future insect control targets and immune-related molecules are described. This study provides valuable basic information that can be used as a gateway to develop new molecular tools for Monochamus alternatus Hope control strategies.