The Effect of Pulsed Radiofrequency Current on Mechanical Allodynia Induced with Resiniferatoxin in Rats
The Effect of Pulsed Radiofrequency Current on Mechanical Allodynia Induced with Resiniferatoxin in Rats
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DOI：
10.1213/ane.0b013e3181e9f62f
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发表时间：
2010-09-01
影响因子：
5.7
通讯作者：
Takasaki, Mayumi
中科院分区：
文献类型：
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作者：
Tanaka, Nobuhiko;Yamaga, Masaharu;Takasaki, Mayumi
背景：脉冲射频（PRF）是一种流行的疼痛治疗方式。PRF电流对神经性疼痛的影响尚未得到详细的研究。我们研究了PRF电流对树脂干扰素（RTX）诱导的大鼠机械异位痛的影响，特别是PRF治疗前异位痛持续时间和PRF暴露时间的影响。方法：成年雄性Sprague-Dawley大鼠（体重250 ~ 400 g）在2% ~ 3%七氟醚麻醉下单次腹腔注射RTX （200 μ g/kg）。S-2组大鼠（n = 5）在RTX治疗1周后给予右坐骨神经PRF电流2分钟；M-2组大鼠（n = 6）， RTX治疗3周后PRF电流持续2分钟；L-2组大鼠（n = 7）， RTX治疗5周后PRF电流2分钟；S-4组大鼠（n = 5）， RTX治疗1周后PRF电流持续4分钟；S-6组大鼠（n = 5）， RTX治疗1周后PRF电流持续6分钟；s - 0组大鼠（n = 3），不给予PRF电流。在RTX治疗1周后，在适当的点插入针和电极6分钟。所有大鼠在注射RTX前、注射RTX后每周、PRF治疗后1、2、3、4和5周，用热测试仪评估von Frey丝机械刺激和热刺激的敏感性，用放置和抓握反射评估运动功能。结果：RTX治疗1周后，两组后肢的断爪阈值均显著低于RTX治疗前的基线。在S-2、S-4、S-6和M-2组，PRF手术后，同侧足爪退出阈值显著增加。经prf治疗和未经prf治疗的后腿之间的差异有统计学意义。S-2组PRF术后同侧和对侧足爪退出阈值显著高于M-2和L-2组。在M-2组和L-2组之间，PRF程序后1、2、4和5周发现显著差异。PRF术后5周，S-6组的同侧对侧足爪退出阈值显著高于S-2和S-4组。S-2组与S-4组在任何时候均无显著差异。在PRF程序后，各组热刺激后的戒断潜伏期和任何时间的运动障碍均无差异。结论：PRF治疗在大鼠机械异常性疼痛早期（1周）应用更有效。将PRF电流暴露时间从2分钟增加到6分钟，显示出显著的抗变动力作用，且无运动损伤。我们建议在出现异常性疼痛时，尽快在神经附近应用PRF电流6分钟。（Anesth Analg 2010;111:784-90）
BACKGROUND: Pulsed radiofrequency (PRF) is a popular pain treatment modality. The effect of PRF current on neuropathic pain has not been examined in detail. We investigated the effect of PRF current on mechanical allodynia induced with resiniferatoxin (RTX) in rats, especially regarding the influence of the duration of allodynia before PRF procedures and that of exposure time to PRF.METHODS: Adult male Sprague-Dawley rats (weighing 250-400 g) received a single intraperitoneal injection of RTX (200 mu g/kg) under 2% to 3% sevoflurane anesthesia. Rats in group S-2 (n = 5) were assigned to receive PRF current to the right sciatic nerve for 2 minutes 1 week after RTX treatment; rats in group M-2 (n = 6), PRF current for 2 minutes 3 weeks after RTX treatment; rats in group L-2 (n = 7), PRF current for 2 minutes 5 weeks after RTX treatment; rats in group S-4 (n = 5), PRF current for 4 minutes 1 week after RTX treatment; rats in group S-6 (n = 5), PRF current for 6 minutes 1 week after RTX treatment; and rats in group S-o (n = 3), no PRF current was delivered. Instead, the needle and electrode were inserted at proper points for 6 minutes 1 week after RTX treatment. All rats were evaluated for sensitivity to mechanical stimulation with von Frey filaments and to thermal stimulation with a thermal testing apparatus and for motor function using placing and grasping reflexes before injection of RTX, every week after injection of RTX, and 1, 2, 3, 4, and 5 weeks after PRF treatment.RESULTS: The paw withdrawal thresholds of both hindpaws 1 week after RTX treatment were significantly lower than the pre-RTX baseline in all groups. In groups S-2, S-4, S-6, and M-2, after PRF procedures, the ipsilateral paw withdrawal thresholds significantly increased. A statistically significant difference was detected between the PRF-treated and PRF-untreated hindpaws. The ipsilateral-contralateral paw withdrawal thresholds after PRF procedures in group S-2 were significantly higher than those in groups M-2 and L-2. Between groups M-2 and L-2, significant differences were found 1, 2, 4, and 5 weeks after PRF procedures. The ipsilateral contralateral paw withdrawal thresholds in group S-6 were significantly higher than those in groups S-2 and S-4 5 weeks after PRF procedures. No significant difference was found between groups S-2 and S-4 at any time. After PRF procedures, no difference in the withdrawal latency after heat stimulation and no motor disturbance were observed at any time in all groups.CONCLUSIONS: PRF treatment was more effective when applied in the early stages of mechanical allodynia (1 week) in rats. Increased exposure time to PRF current from 2 to 6 minutes showed a significant antiallodynic effect without motor impairment. We propose the application of PRF current for 6 minutes adjacent to the nerve as soon as possible when allodynia appears. (Anesth Analg 2010;111:784-90)