EFFECT OF AMINOACYL-4-AMINOHEXOSYL-CYTOSINE GROUP OF ANTIBIOTICS ON RIBOSOMAL PEPTIDYL TRANSFERASE
EFFECT OF AMINOACYL-4-AMINOHEXOSYL-CYTOSINE GROUP OF ANTIBIOTICS ON RIBOSOMAL PEPTIDYL TRANSFERASE
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DOI：
10.1016/0014-5793(73)80639-8
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发表时间：
1973-01-01
期刊：
影响因子：
3.5
通讯作者：
LICHTENTHALER, FW
中科院分区：
文献类型：
--
作者：
CERNA, J;RYCHLIK, I;LICHTENTHALER, FW
图2.抗碘剂I-5011对IacLe-u-pent 3 nu ~ tidt ~ 5th~ ro~的Gzgmcnt反应的影响。React是一种含有核糖体（100 pz蛋白）和Acf-1 Leu-ptwt ~-nuclcotide（1100 cpm）的混合物。Log hf，-抗生素浓度（基于加入甲醇后的tin 31体积计算）。$6，Ackeu-puaomwin地层作为5个对照，没有抑制剂（约900 cpm转移）。从完整的PE~ ti~ y~-*~~ A是！具有两个施主衬底的CS&D，（I+.），- tRNA（图1A）和A&he-tRNA（图1 S）。普奥霉素与这些供体的反应发生在70 S核糖体和的存在下。合适的信使RNA。所有抗生素都能抑制tSe酰氨酰基残基转移到嘌呤霉素上，以HSIastfcidin S最有效，而以pluacetirr最有效，其抑制作用随顺序h~（st）jd~ nS、gougerastin、amicetin= bamicetin（L-5）而减弱，并与供体底物的性质有关。
Fig. 2_ Effect of the antl% iotics l-5 011 the Gzgmcnt reaction of _iacLe-u-pent3nu~~~ otidt~ 5th~~ ro~~~~ _ React&a mixeu_w contained ribosomes (100 pz protein) and Acf “% ILeu-ptwt~-nuclcotide (1100 cpm); otlwr components of the rcnctiun x15xiurc, and procedure were descoibed elsewhere [PJ. Log hf,-oncentmtion of antibiotics (calculated on tfie basis of tin31 volume after addition of methanol). $6, Ackeu-puaomwin formation as 5 control without inhibitors (about 900 cpm transferred). from intact pe~ ti~ y~-*~~ A was! cs&d with two donor substrates,(I+.),-tRNA(fig_ IA) and A&he-tRNA (fig_ IS). Puaomycin reaction with these donors occurs on& in the presence of 70 S ribosorms and of the. appropriate messenger RNA. All antibiotics tested iuhibited the transfer of tSe acylaminoacyl residue to purornycin, HSIastfcidin S was the most effectiz, pliuacetirr was allmost hefiective, The inhibitory effect decreased iv the sequence h~~ st~~ jd~ n S, gougerutin, amicetin= bamicctin (L-5, respectively) and was found to depend on the nature of the donor substrate.