The circular RNA aplacirc_13267 upregulates duck granulosa cell apoptosis by the apla-miR-1-13/THBS1 signaling pathway
The circular RNA aplacirc_13267 upregulates duck granulosa cell apoptosis by the apla-miR-1-13/THBS1 signaling pathway
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环状RNA aplacirc_13267通过apla-miR-1-13/THBS1信号通路上调鸭颗粒细胞凋亡
DOI：
10.1002/jcp.29509
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发表时间：
2020-01-22
影响因子：
5.6
通讯作者：
Du，Jinping
中科院分区：
文献类型：
--
作者：
Wu，Yan;Xiao，Hongwei;Du，Jinping
卵泡发育是决定家禽繁殖性能的关键因素。现有证据表明，环状rna （circRNAs）在多种生物学过程中发挥重要作用，尤其是在转录后调控中，但关于环状rna在鸭卵泡发育中的调控机制鲜有报道。为了更好地探索鸭子卵泡发育的分子机制，我们对卵泡环状rna进行了测序和分析；在鸭卵泡中预测了4204个环状rna。14个环状rna在白色卵泡和黄色卵泡之间差异表达。我们的研究结果表明，aplacirc_013267促进了鸭GCs细胞的凋亡。此外，一项生物信息学预测分析表明，aplacirc_013267参与了circRNA - miRNA - mRNA共表达网络，并通过荧光素酶活性测定观察到aplacirc_013267可以吸收两个与滤泡相关的miRNA。此外，我们发现过表达aplacirc_013267可显著增加血栓反应蛋白- 1 （THBS1）的表达，并下调颗粒细胞凋亡。机制研究表明，aplacirc_013267直接结合并抑制apla‐mir‐1‐13；然后，aplacirc_013267增加THBS1的表达，上调颗粒细胞凋亡。综上所述，我们的研究结果表明，环状rna在鸭卵巢卵泡中具有潜在的作用，并且环状rna可能代表了了解卵泡发育的新途径。
Follicle development is a key factor that determines the reproductive performance of poultry. The existing evidence suggests that circular RNAs (circRNAs) play an important role in a variety of biological processes, especially in posttranscriptional regulation, but the regulatory mechanism of circRNAs in duck follicle development has rarely been reported. To better explore the molecular mechanism of follicle development in ducks, we sequenced and analyzed the follicular circRNAs; 4,204 circRNAs were predicted in the duck follicles. Fourteen circRNAs were differentially expressed between the white follicles and yellow follicles. The results of our studies showed that aplacirc_013267 promoted cell apoptosis in duck GCs. Moreover, a bioinformatics prediction analysis demonstrated that aplacirc_013267 was involved in a circRNA‐miRNA‐mRNA coexpression network and was observed to sponge two follicle‐related miRNAs by a luciferase activity assay. Furthermore, we found that overexpression of aplacirc_013267 significantly increased thrombospondin‐1 (THBS1) expression and downregulated granulosa cell apoptosis. The mechanistic study showed that aplacirc_013267 directly binds to and inhibits apla‐mir‐1‐13; then, aplacirc_013267 increases the expression of THBS1 and upregulates granulosa cell apoptosis. Taken together, our findings demonstrate that circRNAs have potential effects in duck ovarian follicles and that circRNAs may represent a new avenue to understand follicular development.