Characterization of Bacillus subtilis from gastrointestinal tract of hybrid Hulong grouper (Epinephelus fuscoguttatus × E. lanceolatus) and its effects as probiotic additives.
Characterization of Bacillus subtilis from gastrointestinal tract of hybrid Hulong grouper (Epinephelus fuscoguttatus × E. lanceolatus) and its effects as probiotic additives.
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杂交虎纹石斑鱼胃肠道枯草芽孢杆菌的特性及其作为益生菌添加剂的作用
DOI：
10.1016/j.fsi.2018.10.058
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发表时间：
2019-01
影响因子：
4.7
通讯作者：
Sheng Zhou;D. Song;Xiaofeng Zhou;Xinliang Mao;Xuefeng Zhou;Sunli Wang;Jingguang Wei;Youhua Huang-Youhua
中科院分区：
文献类型：
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作者：
Sheng Zhou;D. Song;Xiaofeng Zhou;Xinliang Mao;Xuefeng Zhou;Sunli Wang;Jingguang Wei;Youhua Huang-Youhua
益生菌被广泛用于改善动物的生长和健康。然而，海洋水产益生菌在中国的应用和许可很少。在本研究中，a Bacillusspp.从湖龙石斑鱼胃肠道中分离到一株枯草芽孢杆菌，经鉴定为一株新的枯草芽孢杆菌，命名为7 k。B。枯草杆菌7 k具有良好的产孢能力和耐热性，对模拟胃液和模拟十二指肠液的耐受性较好，显示了其作为益生菌的潜力。在B.枯草杆菌饲料中添加枯草杆菌7 k促进了虎龙石斑鱼的生长。而且，B。枯草杆菌7 k能抑制虹彩病毒的感染，使B. subtilis 7 k是一种适合于维持鱼类健康的益生菌。我们的研究结果还表明，B. subtilis 7 k在病毒感染下诱导了湖龙石斑鱼的非特异性免疫应答。用含B. 108和1010 cfu g-1的枯草杆菌7 k饲喂4-8周后，与对照组相比，血清溶菌酶活性、血清替代补体活性（ACH 50）、血清杀菌活性、呼吸爆发、超氧化物歧化酶活性（SOD）和头肾白细胞吞噬活性显著增强。总之，B.枯草杆菌7 K是一种具有持久性、促生长、增强免疫力和保健功能的石斑鱼益生菌。
Probiotics are widely used for the improvement of animals' growth and health. However, few marine aquatic probiotics are applied and licensed in China. In this study, aBacillusspp. strain was isolated from the Hulong grouper gastrointestinal tract, which was identified as a new strain of Bacillus subtilis and was named as 7k. B. subtilis7k showed desirable capability of sporulation and resistance to heat, simulated gastric juice and simulated duodenum juice, indicating its potential as probiotics. Seven antimicrobial chemicals were found in the secretion of theB. subtilis7k.B. subtilis7k addition in diet promoted the growth rate of Hulong groupers. Moreover,B. subtilis7k can inhibit infection by iridovirus, makingB. subtilis7k a suitable kind of probiotic for maintaining fishes’ health. Our results also revealed thatB. subtilis7k induced non-specific immune response in Hulong grouper under virus infection. Hulong grouper fed by diets containingB. subtilis7k at 108and 1010cfu g−1for 4–8 weeks were significantly strengthened in serum lysozyme activity, serum alternative complement activity (ACH50), serum bactericidal activity, respiratory burst, superoxide dismutase activity (SOD), and phagocytic activity of head kidney leucocytes when compared with those fed by control diets. In conclusion,B. subtilis7k was isolated and characterized to be a kind of process enduring, growth stimulating, immunity enhancing and health promoting probiotic using in grouper culture.