Urea-induced release of heat-labile enterotoxin from Escherichia coli.
Urea-induced release of heat-labile enterotoxin from Escherichia coli.
复制标题
尿素诱导大肠杆菌释放不耐热肠毒素。
DOI：
10.1128/jcm.29.4.773-777.1991
复制
发表时间：
1991
影响因子：
9.4
通讯作者：
Finkelstein,RA
中科院分区：
文献类型：
--
作者：
Qu,ZH;Boesman-Finkelstein,M;Finkelstein,RA
Urea induces the release of heat-labile enterotoxin (LT) from cells of LT-producing Escherichia coli strains. Optimal conditions were defined by using the checkerboard immunoblotting system. LT release was highest when E. coli cells were incubated in 8 M urea, pH 8.0, at 37 degrees C in a water bath for 30 min. Urea was more effective than polymyxin B in inducing the release of LT antigen from E. coli; the activity of LT from urea-treated cells was seven times that of LT from polymyxin B-treated cells. Urea also increased the antigenic and biological reactivities of purified LT. This procedure is potentially applicable for the detection of LT-producing E. coli strains in the clinical laboratory.
