Enhanced production of Pacific dulse (Palmaria mollis) for co-culture with abalone in a land-based system: nitrogen, phosphorus, and trace metal nutrition
Enhanced production of Pacific dulse (Palmaria mollis) for co-culture with abalone in a land-based system: nitrogen, phosphorus, and trace metal nutrition
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DOI：
10.1016/j.aquaculture.2003.09.010
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发表时间：
2004-06
期刊：
影响因子：
4.5
通讯作者：
C. Demetropoulos;C. Langdon
中科院分区：
文献类型：
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作者：
C. Demetropoulos;C. Langdon
太平洋鲍鱼（Palmaria mollis）是红鲍鱼（Haliotis rufescens）和日本鲍鱼（Haliotis discus hannai）的优质藻类饲料；然而，能够生产商业数量的软体霉的培养技术仍在发展中。本研究旨在提高我们对集约化陆基沼地沼地沼地生产的养分需求和管理策略的认识。所有实验均在24-52 mol光子m−2day−1的中高人工光和低海水交换（1 vol day−1）条件下进行，以尽量减少海水泵送成本。每5 ~ 7 d施用养分与每日施用相比，对软体藻生长无显著影响。与光照周期相比，在暗周期添加营养物质对附生真菌的控制效果更好。中微量金属（Gran）的添加。可以。J. Microbiol. 8(1962) 229)与仅提供硝酸盐和磷酸盐的培养物相比，显著提高了生长速率。将Zn浓度增加到与Mn浓度相等（1.37 μM day−1），提高了产率。在长期生长（9周）中，nano3作为氮源优于NH4NO3，尽管NH4NO3在短期生长（前2-5周）中优于NH4NO3。这可能是由于实验设计造成的NH4+ -N毒性。添加1176 ~ 2942 μM day−1NO3−-N（平均日氮负荷量）对褐藻生长无显著影响，但在添加2942 μM day−1NO3−-N时，褐藻生长呈增加趋势。当磷添加量超过83.3 μM day−1（平均日磷负荷）时，P. mollis的生长急剧下降。组织养分浓度和摩尔比（N、P、微量金属、N/P和C/N）可用于制定最佳营养管理策略，以确保最大的脉冲生长速率。
Pacific dulse (Palmaria mollis) is a high-quality algal feed for both red abalone (Haliotis rufescens) and Japanese abalone (Haliotis discus hannai); however, culture techniques capable of producing commercial quantities of P. mollis are still in development. This study was undertaken to improve our understanding of nutrient requirements and management strategies for P. mollis production in intensive, land-based systems. All experiments were conducted under moderate to high artificial light of 24–52 mol photons m−2day−1and low seawater exchange (1 vol day−1), in order to minimize seawater pumping costs. Application of nutrients every 5–7 days resulted in no significant difference in P. mollis growth compared with daily applications. Nutrient additions during the dark cycle compared with the light cycle were found to be effective in controlling epiphytes. The addition of f medium trace metals (Gran. Can. J. Microbiol. 8 (1962) 229) significantly increased growth rates compared with those of cultures supplied with just nitrate and phosphate alone. The concentration of Zn was increased to equal that of Mn (1.37 μM day−1), with resulting improvement in yields. NaNO3as a source of nitrogen was found to be superior for long-term growth (9 weeks) compared with NH4NO3, although NH4NO3was superior on a short-term basis (first 2–5 weeks). This was likely due to NH4+–N toxicity resulting from the experimental design. While P. mollis growth was not significantly different between additions of 1176–2942 μM day−1NO3−–N (average daily N load), an increasing trend in growth was observed with increasing N loads up to 2942 μM day−1NO3−–N. P. mollis showed a sharp decline in growth with P additions above 83.3 μM day−1(average daily P load). Tissue nutrient concentrations and molar ratios (N, P, trace metals, N/P and C/N) can be used in the development of optimal nutrient management strategies to ensure maximum dulse growth rates.