Adenovirus VAI RNA prevents phosphorylation of the eukaryotic initiation factor 2 alpha subunit subsequent to infection.
Adenovirus VAI RNA prevents phosphorylation of the eukaryotic initiation factor 2 alpha subunit subsequent to infection.
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DOI：
10.1073/pnas.82.13.4321
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发表时间：
1985-07
影响因子：
11.1
通讯作者：
R. Schneider;B. Safer;S. Munemitsu;C. E. Samuel;T. Shenk
中科院分区：
文献类型：
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作者：
R. Schneider;B. Safer;S. Munemitsu;C. E. Samuel;T. Shenk
腺病毒的病毒相关VAI RNA是一种小的RNA聚合酶III转录的物质，在感染后晚期有效翻译mRNA所需。缺失突变体dl 331不能产生这种RNA，因此生长不良。三条证据表明，VAI RNA通过阻止真核起始因子2（eIF-2）功能的失活来促进翻译。首先，突变体的翻译缺陷可以通过添加eIF-2或eIF-2B（GTP再循环因子）来缓解。第二，mu感染细胞的提取物表现出增强的蛋白P1/eIF-2 α亚基激酶活性。第三，dl 331可以在不表达激酶的细胞中以几乎正常的动力学生长。
The virus-associated VAI RNA of adenovirus is a small, RNA polymerase III-transcribed species required for efficient translation of mRNAs late after infection. Deletion mutant dl331 fails to produce this RNA and, as a result, grows poorly. Three lines of evidence suggest that VAI RNA facilitates translation by preventing inactivation of the function of eukaryotic initiation factor 2 (eIF-2). First, the mutant's translational defect can be relieved by addition of eIF-2 or eIF-2B (GTP recycling factor). Second, extracts of mutant-infected cells exhibit enhanced protein P1/eIF-2 alpha subunit kinase activity. Third, dl331 can grow with nearly normal kinetics in cells that do not express the kinase.