Stimulation of formation of cAMP by 5-hydroxytryptamine in myenteric ganglia isolated from guinea pig small intestine.
Stimulation of formation of cAMP by 5-hydroxytryptamine in myenteric ganglia isolated from guinea pig small intestine.
复制标题
5-羟色胺刺激豚鼠小肠肌间神经节中 cAMP 的形成。
DOI：
10.1016/0024-3205(94)00675-x
复制
发表时间：
1994
期刊：
影响因子：
6.1
通讯作者：
Wood,JD
中科院分区：
文献类型：
--
作者：
Xia,Y;Fertel,RH;Wood,JD
本实验观察了5-羟色胺（5-HT）及其激动剂和拮抗剂对豚鼠小肠肌间神经丛酶解神经节cAMP生成的影响。5-HT可刺激cAMP的生成，并呈剂量和时间依赖性. 5-HT的兴奋作用可被5-HT 1 P拮抗剂伦扎必利所抑制，而不被5-HT 3拮抗剂托烷司琼（原ICS 205-930）所抑制。伦扎必利和西沙必利均不增加cAMP水平。浓度大于1.0 μM的托烷司琼可抑制cAMP水平。cAMP水平不受5-HT 3激动剂2-甲基-5-羟色胺的影响。一种假定的5-HT 4受体激动剂，5-甲氧基色胺，刺激cAMP的形成，但在较小程度上比5-HT。我们的结论是，5-HT的行为刺激腺苷酸环化酶和cAMP的形成在肌间神经节。5-HT 1 P受体是最可能参与5-HT对cAMP形成作用的亚型。
Effects of 5-hydroxytryptamine (5-HT) and related agonists and antagonists on formation of cAMP were determined for enzymatically dissociated ganglia from the myenteric plexus of the guinea-pig small intestine. Formation of cAMP was stimulated by 5-HT in both dose- and time- dependent manners. The stimulatory action of 5-HT was suppressed by the 5-HT1Pantagonist, renzapride, but not by the 5-HT3antagonist, tropisetron (formerly ICS 205–930). Neither renzapride nor cisapride increased the levels of cAMP. Levels of cAMP were suppressed by concentrations of tropisetron greater than 1.0 μM. Levels of cAMP were unaffected by the 5-HT3agonist, 2-methyl-5-hydroxytryptamine. A putative 5-HT4receptor agonist, 5-methoxytryptamine, stimulated formation of cAMP, but to a lesser extent than 5-HT. We conclude that 5-HT acts to stimulate adenylate cyclase and the formation of cAMP in myenteric ganglia. The 5-HT1Preceptor is the most likely subtype involved in 5-HT action on cAMP formation.