Syringolin, a novel peptide elicitor from Pseudomonas syringae pv. syringae that induces resistance to Pyricularia oryzae in rice
Syringolin, a novel peptide elicitor from Pseudomonas syringae pv. syringae that induces resistance to Pyricularia oryzae in rice
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DOI：
10.1094/mpmi.1998.11.8.727
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发表时间：
1998-08-01
影响因子：
3.5
通讯作者：
Dudler, R
中科院分区：
文献类型：
--
作者：
Wäspi, U;Blanc, D;Dudler, R
水稻（Oryza sativa）对非寄主病原体丁香假单胞菌（Pseudomonas syringae pv, syringae）的识别导致积极反应，最终导致对稻瘟病真菌稻瘟病菌（pyricaria oryzae）的局部获得性抗性。这种防御反应的一个可观察到的方面是一组转录本的丰度增加。其中一种转录本Pir7b的积累依赖于细菌lemA基因的功能，该基因编码双组分调控系统的一部分。这表明lemA调控系统控制了Pir7b启动子的产生，转录物积累。对该激发子进行了纯化，并对其结构进行了分析。丁香素是一种新型的、不寻常的分泌肽，由两个非蛋白氨基酸5-甲基-4-氨基-2-己烯酸和3,4-脱氢赖氨酸组成的12元环组成。后者的a-氨基通过肽键连接到一个缬氨酸，而这个缬氨酸又通过尿素片段连接到另一个缬氨酸。紫丁香素在水稻叶片上的应用引发了Pir7b以及其他防御相关转录物的积累，并诱导了对P. oryzae的抗性。因此，紫丁香素是水稻能够感知非寄主病原体紫丁香假单胞菌的几个决定因素之一。
Recognition by rice plants (Oryza sativa) of the nonhost pathogen Pseudomonas syringae pv, syringae leads to an active response ultimately resulting in local acquired resistance against the rice blast fungus Pyricularia oryzae, An observable aspect of this defense response is the increased abundance of a set of transcripts. The accumulation of one of these transcripts, Pir7b was dependent on the function of the bacterial lemA gene, which encodes part of a two-component regulatory system. This suggested that the lemA regulatory system controlled the production of an elicitor of Pir7b, transcript accumulation. This elicitor, which we name syringolin, was purified to homogeneity and its structure was elucidated. Syringolin is a novel and unusual secreted peptide consisting of a 12-membered ring formed by the two non-proteinogenic amino acids 5-methyl-4-amino-2-hexenoic acid and 3,4-dehydrolysine. The a-amino group of the latter is connected by a peptide bond to a valine that in turn is linked to a second valine via a urea moiety, Application of syringolin onto rice leaves elicited the accumulation of Pir7b as well as of other defense-related transcripts and induced resistance toward P. oryzae. Thus, syringolin is one of several determinants by which rice plants can perceive the nonhost pathogen P. syringae pv, syringae.