Connexin 37 is localized in renal epithelia and responds to changes in dietary salt intake.
Connexin 37 is localized in renal epithelia and responds to changes in dietary salt intake.
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DOI：
10.1152/ajprenal.00295.2009
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
A. Stoessel;N. Himmerkus;M. Bleich;S. Bachmann;F. Theilig
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文献类型：
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作者：
A. Stoessel;N. Himmerkus;M. Bleich;S. Bachmann;F. Theilig
缝隙连接蛋白是缝隙连接通道的主要组成部分，在细胞间通讯中起重要作用。在肾脏中，已经鉴定了连接蛋白（Cx）家族的几个成员。到目前为止，已经显示了Cx 37、Cx40和Cx43对肾血管表达和血流动力学的影响。此外，Cx 30、Cx30.3和Cx43已被鉴定为肾小管上皮间隙连接和/或半通道的一部分。然而，其他Cx家族成员在肾上皮结构中的定位和作用仍不确定。我们的目的是本地化Cx 37在肾脏上皮细胞的表达和潜在的功能，以获得信息。啮齿动物肾脏的免疫组织化学显示血管和沿着肾单位的特征性点状图案。在粗的升支和远曲小管中发现强基底侧表达。在近端小管和集合管中也发现了较弱的丰度。原位杂交和实时PCR分离的肾单位片段证实了这种分布在mRNA水平。超微结构上，Cx 37免疫染色仅限于基底外侧细胞交错和内折。作为一种功能性方法，大鼠被喂食低盐或高盐饮食。与对照组和高盐饮食组相比，低盐饮食组大鼠Cx 37 mRNA和蛋白水平显著升高。这可能表明肾小管对钠重吸收变化的适应性反应。总之，肾上皮细胞在其基底外侧膜中表达Cx 37。在这里，Cx 37缝隙连接的形成可能参与细胞通讯和调节上皮细胞的载体运输。
Connexins are the main components of gap junction channels, which are important for intercellular communication. In the kidney, several members of the connexin (Cx) family have been identified. Renal vascular expression and hemodynamic impacts have so far been shown for Cx37, Cx40, and Cx43. Additionally, Cx30, Cx30.3, and Cx43 have been identified to be part of tubular epithelial gap junctions and/or hemichannels. However, the localization and role of other Cx family members in renal epithelial structures remain undetermined. We aimed to localize Cx37 in the kidney to obtain information on its epithelial expression and potential functions. Immunohistochemistry in rodent kidney showed characteristic punctate patterns in the vasculature and along the nephron. Strong basolateral expression was found in the thick ascending limb and distal convoluted tubule. Weaker abundances were found in the proximal tubule and the collecting duct also at the basolateral side. In situ hybridization and real-time PCR of isolated nephron segments confirmed this distribution at the mRNA level. Ultrastructurally, Cx37 immunostaining was confined to basolateral cell interdigitations and infoldings. As a functional approach, rats were fed low- or high-salt diets. Compared with control and high-salt diets, rats treated with low-salt diet showed significantly increased Cx37 mRNA and protein levels. This may be indicative of an adaptive tubular response to changes in sodium reabsorption. In summary, renal epithelia express Cx37 in their basolateral membranes. Here, the formation of Cx37 gap junctions may be involved in cellular communication and adjustments of vectorial epithelial transport.