PHENOTYPIC MARKER EXPRESSION DURING FETAL AND NEONATAL DIFFERENTIATION OF RAT TRACHEAL EPITHELIAL-CELLS
PHENOTYPIC MARKER EXPRESSION DURING FETAL AND NEONATAL DIFFERENTIATION OF RAT TRACHEAL EPITHELIAL-CELLS
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DOI：
10.1165/ajrcmb/8.5.546
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发表时间：
1993-05-01
影响因子：
6.4
通讯作者：
NETTESHEIM, P
中科院分区：
文献类型：
--
作者：
RANDELL, SH;SHIMIZU, T;NETTESHEIM, P
在大鼠气管上皮（RTE）细胞的胚胎和新生儿分化过程中的表型标记的表达进行了检查。人角蛋白18的大鼠对应物主要存在于成人气管的柱状细胞中。在妊娠第12天（妊娠期= 21.5天）首次观察到的原始气管上皮中检测到。染色强度逐渐增加，到GD 17时，主要位于上皮的顶端部分。GD 13时，在气管中几乎检测不到人角蛋白19的大鼠对应物，但在GD 19时和GD 19后，几乎所有RTE细胞中均大量存在。形态学和免疫细胞化学鉴定的分泌和纤毛细胞出现在GD 18。从GD 19到出生后第1天，纤毛细胞数量缓慢上升，而分泌细胞数量急剧增加。分泌颗粒抗原检测的单克隆抗体RTE 9和11是罕见的成人气管，但几乎所有的围产期分泌细胞中高度表达。与此相反，单克隆抗体RTE 12，这是目前在所有成人气管表面分泌细胞检测到的表位，直到出生后第1天才出现，并缓慢增加。这些结果表明，在发育和出生后成熟的分泌细胞的生化组成的显着变化。对于上述分子，在气管移植物中的上皮再生期间观察到类似的表达模式（Am. J.呼吸。细胞分子1992; 7：30-41）。在GD 20首次观察到上皮和基底细胞的假分层。角蛋白14，这是局限于基底细胞在正常成人气管，不存在于新生的基底细胞，但出生后第1天后出现。与本研究结果相反，在气管移植物上皮再生过程中，角蛋白14出现在高度分化的分泌细胞或纤毛细胞的标记之前。因此，再生过程中细胞分化的生化序列并不能精确地再现发育过程。
The expression of phenotypic markers was examined during fetal and neonatal differentiation of rat tracheal epithelial (RTE) cells. The rat counterpart of human keratin 18 was predominantly found in columnar cells in the adult trachea. It was detected in the primordial tracheal epithelium first seen on gestational day (GD) 12 (term = 21.5 days). Staining intensity gradually increased, and by GD 17 it was principally localized to the apical portion of the epithelium. The rat counterpart of human keratin 19 was barely detectable in the trachea on GD 13 but became abundant in almost all RTE cells on and after GD 19. Morphologically and immunocytochemically identifiable secretory and ciliated cells appeared on GD 18. Ciliated cell number slowly rose while secretory cells increased dramatically on GD 19 through postnatal day 1. The secretory granule antigens detected by monoclonal antibodies RTE 9 and 11 were rare in the adult trachea but were highly expressed in virtually all of the perinatal secretory cells. In contrast, the epitope detected by monoclonal antibody RTE 12, which was present in all adult tracheal surface secretory cells, did not appear until postnatal day 1 and slowly increased. These results demonstrate marked shifts in the biochemical composition of secretory cells during development and postnatal maturation. For the above-mentioned molecules, a similar expression pattern was observed during epithelial regeneration in tracheal grafts (Am. J. Respir. Cell Mol. Biol. 1992; 7:30-41). Pseudostratification of the epithelium and basal cells was first observed on GD 20. Keratin 14, which is confined to basal cells in the normal adult trachea, was not present in the nascent basal cells but appeared after postnatal day 1. In contrast to the present results, during epithelial regeneration in tracheal grafts keratin 14 appeared before markers of highly differentiated secretory or ciliated cells. Thus, the biochemical sequence of cellular differentiation during regeneration did not precisely recapitulate development.