The proofreading domain of Escherichia coli DNA polymerase I and other DNA and/or RNA exonuclease domains
The proofreading domain of Escherichia coli DNA polymerase I and other DNA and/or RNA exonuclease domains
复制标题
DOI：
10.1093/nar/25.24.5110
复制
发表时间：
1997-12-15
影响因子：
14.9
通讯作者：
Mian, IS
中科院分区：
文献类型：
--
作者：
Moser, MJ;Holley, WR;Mian, IS
先前的序列分析研究表明，细菌核糖核酸酶（RNase）Ds包含一个完整的结构域，该结构域也存在于智人多发性肌炎-硬皮病重叠综合征100 kDa自身抗原和沃纳综合征蛋白中。该RNase D 3 '->5'核糖核酸外切酶结构域被预测具有与DNA聚合酶的3 '->5'脱氧核糖核酸外切酶（校正）结构域类似的结构和作用机制。在这里，隐马尔可夫模型（HMM）和系统发育研究已被用来确定和测序其他序列，可能拥有这个核酸外切酶域。结果表明，它也存在于RNase T家族;伯氏疏螺旋体P93蛋白，莱姆病中的免疫显性抗原;噬菌体T4 dexA和大肠杆菌外切核酸酶I，降解单链DNA的进行性3 '->5'脱氧核糖核酸酶;枯草芽孢杆菌dinG，一种可能参与DNA修复和可能复制的解旋酶，以及肽合成酶1;酿酒酵母Pab 1 p依赖性多聚腺苷酸核酸酶PAN 2亚基，缩短mRNA多聚腺苷酸尾所需;秀丽隐杆线虫和小家鼠CAF 1，转录因子CCR 4相关因子1;非洲爪蟾XPMC 2，预防裂殖酵母中的有丝分裂灾难;黑腹果蝇平等，卵母细胞的规格和轴确定，和exuperantia，卵母细胞极性的建立;智人HEM 45，在肿瘤细胞系和子宫中表达，并受雌激素调节;和31个开放阅读框架，包括詹氏甲烷球菌中的一个。多序列比对和校对结构域的两个三维结构的检查允许定义该核酸外切酶结构域的核心序列、结构和功能元件。
Prior sequence analysis studies have suggested that bacterial ribonuclease (RNase) Ds comprise a complete domain that is found also in Homo sapiens polymyositis-scleroderma overlap syndrome 100 kDa autoantigen and Werner syndrome protein. This RNase D 3'-->5' exoribonuclease domain was predicted to have a structure and mechanism of action similar to the 3'-->5' exodeoxyibonuclease (proofreading) domain of DNA polymerases. Here, hidden Markov model (HMM) and phylogenetic studies have been used to identify and characterise other sequences that may possess this exonuclease domain. Results indicate that it is also present in the RNase T family; Borrelia burgdorferi P93 protein, an immunodominant antigen in Lyme disease; bacteriophage T4 dexA and Escherichia coli exonuclease I, processive 3'-->5' exodeoxyribonucleases that degrade single-stranded DNA; Bacillus subtilis dinG, a probable helicase involved in DNA repair and possibly replication, and peptide synthase 1; Saccharomyces cerevisiae Pab1p-dependent poly(A) nuclease PAN2 subunit, required for shortening mRNA poly(A) tails; Caenorhabditis elegans and Mus musculus CAF1, transcription factor CCR4-associated factor 1; Xenopus laevis XPMC2, prevention of mitotic catastrophe in fission yeast; Drosophila melanogaster egalitarian, oocyte specification and axis determination, and exuperantia, establishment of oocyte polarity; H.sapiens HEM45, expressed in tumour cell lines and uterus and regulated by oestrogen; and 31 open reading frames including one in Methanococcus jannaschii. Examination of a multiple sequence alignment and two three-dimensional structures of proofreading domains has allowed definition of the core sequence, structural and functional elements of this exonuclease domain.