Developing fluoromodule-based probes for in vivo monitoring the bacterial infections and antibiotic responses
Developing fluoromodule-based probes for in vivo monitoring the bacterial infections and antibiotic responses
复制标题
开发基于氟模块的探针用于体内监测细菌感染和抗生素反应
DOI：
10.1016/j.talanta.2021.122610
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发表时间：
2021-06-18
期刊：
影响因子：
6.1
通讯作者：
Hu，Hai-Yu
中科院分区：
文献类型：
--
作者：
Wang，Xiang;Wang，Qinghua;Hu，Hai-Yu
最近，抗生素耐药性已成为严重的公共卫生问题，这需要开发新一代抗生素。药效评价在药物发现过程中至关重要。尽管现在有许多先进的成像系统可用，但细菌感染的敏感体内诊断和药物疗效的直接可视化技术仍有待开发。在这项研究中，我们开发了新型近红外（NIR）荧光探针。这些探针在溶液中呈黑色，但在与同源报告分子荧光激活蛋白 (FAP) 结合时发出高荧光。我们利用重组表达的FAP_dH6.2大肠杆菌建立了体内细菌感染模型。 coliand 应用这种基于近红外荧光模块的系统来诊断细菌感染并通过经典的膜裂解机制监测疾病进展及其对某种抗生素的反应。这种基于近红外荧光模块的系统将发现有关细菌感染的新信息并识别更新的抗菌实体。
Recently, antibiotic resistant has become a serious public health concern, which warrants new generations of antibiotics to be developed. Pharmacodynamic evaluation is crucial in drug discovery processes. Despite numerous advanced imaging systems are available nowadays, technologies for the sensitivein vivodiagnosis of bacterial infections and direct visualization of drug efficacy are yet to be developed. In this study, we have developed novel near-infrared (NIR) fluorogenic probes. These probes are dark in solution but highly fluorescent when bound to the cognate reporter, fluorogen-activating protein (FAP). We established thein vivobacterial infection model using FAP_dH6.2 recombinantly expressedE. coliand applied this NIR fluoromodule-based system for diagnosing bacterial infections and monitoring disease progressions and its responses to a type of antibiotics through classic mechanism of membrane lysis. This NIR fluoromodule-based system will discover new information on bacterial infections and identify newer antibacterial entities.