The effects of Runx2 immobilization on poly (epsilon-caprolactone) on osteoblast differentiation of bone marrow stromal cells in vitro.
The effects of Runx2 immobilization on poly (epsilon-caprolactone) on osteoblast differentiation of bone marrow stromal cells in vitro.
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DOI：
10.1016/j.biomaterials.2010.01.029
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发表时间：
2010-04
期刊：
影响因子：
14
通讯作者：
Krebsbach, Paul H.
中科院分区：
文献类型：
--
作者：
Zhang, Ying;Deng, Xiaopei;Scheller, Erica L.;Kwon, Tae-Geon;Lahann, Joerg;Franceschi, Renny T.;Krebsbach, Paul H.
关键词：
体内再生基因治疗是一种有前途的骨再生方法，可以通过手术植入骨诱导材料和随后招募宿主来源的细胞来帮助解决细胞来源的限制。局部病毒递送可以降低病毒分散的风险，增强转导效率，并减少给药/注射剂量，从而提高患者的安全性。在这篇手稿中，我们提出了一个定制的策略，通过使用反应性聚合物涂层，以提高在体外骨髓基质细胞（BMSCs）的成骨细胞分化的腺病毒表达的runt相关转录因子2（AdRunx 2）。采用化学气相沉积（CVD）技术在聚己内酯（PCL）表面制备了一层具有胺活性酯基的聚对苯二甲撑羧酸五氟苯酚酯-对苯二甲撑共聚物薄膜，并通过酰胺键将抗腺病毒抗体偶联到该薄膜上。在抗体缀合后，AdRunx 2通过抗体-抗原相互作用缀合至PCL表面。骨髓间充质干细胞在成骨培养基中诱导成骨细胞分化。碱性磷酸酶（ALP）活性、钙沉积和基质矿化被证实为成骨细胞形成的标志物。与对照组相比，在AdRunx 2修饰的PCL存在下孵育BMSC导致ALP活性增加6.5倍，基质矿化显著增加。
In vivo regenerative gene therapy is a promising approach for bone regeneration and can help to address cell-source limitations through surgical implantation of osteoinductive materials and subsequent recruitment of host-derived cells. Localized viral delivery may reduce risk of virus dispersion, enhance transduction efficiency, and reduce administration/injection dosing, which subsequently increases patient safety. In this manuscript, we present a custom-tailored strategy to immobilize adenovirus expressing runt-related transcription factor 2 (AdRunx2) by using reactive polymer coatings to enhance in vitro osteoblast differentiation of bone marrow stromal cells (BMSCs). A thin polymer film of poly[p-xylylene carboxylic acid pentafluorophenol ester-co-p-xylylene] equipped with amine-reactive active ester groups was deposited on the surface of poly (ε-caprolactone) (PCL) using the CVD polymerization technique and then anti-adenovirus antibody was conjugated on the material with an amide chemical bond. Following antibody conjugation, AdRunx2 was conjugated to the PCL surface through antibody-antigen interaction. Osteoblast differentiation of BMSCs was induced by incubation in osteogenic medium. Alkaline phosphatase (ALP) activity, calcium deposition, and matrix mineralization were confirmed as markers of osteoblast formation. Incubation of the BMSCs in the presence of AdRunx2 modified PCL resulted in a 6.5-fold increase in ALP activity and significant increases in matrix mineralization when compared to controls.
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影响因子：
3.5
作者：
Hu, Wei-Wen;Lang, Michael W.;Krebsbach, Paul H.
通讯作者：
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影响因子：
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作者：
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通讯作者：
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