Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination
Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination
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DOI：
10.1110/ps.04856504
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发表时间：
2004-10-01
期刊：
影响因子：
8
通讯作者：
Song, HW
中科院分区：
文献类型：
--
作者：
Cheng, ZH;Liu, YY;Song, HW
Ski8p 是一种 WD 重复蛋白，在外泌体依赖性 3' 至 5' mRNA 衰变中对 Ski 复合物组装发挥重要作用。此外，Ski8p 通过与 Spo11p 蛋白相互作用参与减数分裂重组。我们以 2.2 埃的分辨率测定了酿酒酵母中 Ski8p 的晶体结构。该结构揭示了 Ski8p 可以折叠成七叶 beta 螺旋桨。对 Ski8p 分子表面上氨基酸的序列保守性和疏水性进行图谱显示，β 螺旋桨顶部表面有一个显着位点，该位点很可能参与介导 Ski8p 与 Ski3p 和 Spo11p 的相互作用。诱变结合酵母双杂交和 GST 下拉分析确定了 β 螺旋桨的顶面是 Ski8p 与 Ski3p 和 Spollp 结合所必需的。讨论了 Ski8p 在 mRNA 衰变和减数分裂重组中的功能意义。
Ski8p is a WD-repeat protein with an essential role for the Ski complex assembly in an exosome-dependent 3'-to-5' mRNA decay. In addition, Ski8p is involved in meiotic recombination by interacting with Spo11p protein. We have determined the crystal structure of Ski8p from Saccharomyces cerevisiae at 2.2 Angstrom resolution. The structure reveals that Ski8p folds into a seven-bladed beta propeller. Mapping sequence conservation and hydrophobicities of amino acids on the molecular surface of Ski8p reveals a prominent site on the top surface of the beta propeller, which is most likely involved in mediating interactions of Ski8p with Ski3p and Spo11p. Mutagenesis combined with yeast two-hybrid and GST pull-down assays identified the top surface of the beta propeller as being required for Ski8p binding to Ski3p and Spollp. The functional implications for Ski8p function in both mRNA decay and meiotic recombination are discussed.