Nocardioides aromaticivorans sp nov., a dibenzofuran-degrading bacterium isolated from dioxin-polluted environments
Nocardioides aromaticivorans sp nov., a dibenzofuran-degrading bacterium isolated from dioxin-polluted environments
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DOI：
10.1016/j.syapm.2004.10.002
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发表时间：
2005-03-01
影响因子：
3.4
通讯作者：
Hiraishi, A
中科院分区：
文献类型：
--
作者：
Kubota, M;Kawahara, K;Hiraishi, A
对从二恶英污染环境中分离得到的7株二苯并呋喃（DF）降解菌进行了鉴定。这些菌株能够以二苯并呋喃作为唯一的碳源和能量源生长。在与二苯并呋喃一起生长的过程中，它们产生了一种可溶性的黄色代谢物，这种代谢物表现出独特的吸收最大值随ph值的变化。二苯并对二恶英和联苯也在色素生产中降解。分离株为严格的好氧和趋化有机营养型，具有革兰氏阳性、无运动的杆状细胞。化学分类分析表明，该细胞的肽聚糖中含有L，L-二氨基苯甲酸，支链脂肪酸是主要的脂肪酸，甲基萘醌MK-8（H-4）是唯一的呼吸醌。菌株DNA的G + C含量在72.0 ~ 72.4mol%之间。分离株的16S rRNA基因序列非常相似（>= 99.8%）。系统发育分析表明，该分离株与诺卡伊蚊属形成一个聚类，以单纯诺卡伊蚊和硝基酚诺卡伊蚊为近邻。DNA杂交研究表明，分离物与诺卡伊蚊属任一种的杂交率均小于55%。在此基础上，提出了新的df降解菌株为芳香诺卡依菌。型应变为H-1 （IAM 14992, JCM 11674, DSM 15131）。(c) 2004 Elsevier GmbH版权所有。
Seven strains of dibenzofuran (DF)-degrading bacteria isolated from dioxin-polluted environments were characterized. These isolates were able to grow with dibenzofuran as the sole carbon and energy source. During the growth with dibenzofuran, they produced a soluble yellow metabolite that exhibited a unique pH-dependent shift of absorption maxima. Dibenzo-p-dioxin and biphenyl were also degraded with pigment production. The isolates were strictly aerobic and chemoorganotrophic and had Gram-positive, nonmotile, rod-shaped cells. Chemotaxonomic analyses showed that cells contained L,L-diaminopimeric acid in the peptidoglycan, branched-chain fatty acids as major fatty acids, and menaquinone MK-8(H-4) as the sole respiratory quinone. The G + C content of the DNA of the isolates ranged from 72.0 to 72.4mol%. The 16S rRNA gene sequences of the isolates were very similar to each other (>= 99.8%). The phylogenetic analysis showed that the isolates formed a cluster with species of the genus Nocardioides with Nocardioides simplex and Nocardioides nitrophenolicus as their nearest neighbors. DNA DNA hybridization studies showed that the isolates showed a hybridization level of less than 55% to any tested species of the genus Nocardioides. Based on these data, Nocardioides aromaticivorans sp. nov. is proposed for the new DF-degrading isolates. The type strain is strain H-1 (IAM 14992, JCM 11674, DSM 15131). (c) 2004 Elsevier GmbH. All rights reserved.