Neural tissue engineering options for peripheral nerve regeneration
Neural tissue engineering options for peripheral nerve regeneration
复制标题
用于周围神经再生的神经组织工程选择
DOI：
10.1016/j.biomaterials.2014.04.064
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发表时间：
2014-08-01
期刊：
影响因子：
14
通讯作者：
Williams, David F.
中科院分区：
文献类型：
--
作者：
Gu, Xiaosong;Ding, Fei;Williams, David F.
组织工程神经移植物（TENG）已成为自体神经移植物的潜在替代品，自体神经移植物是周围神经修复的金标准。通常，TENG由包含生物化学线索的基于生物材料的模板组成。许多TENG已在实验上用于桥接各种动物模型中的长外周神经间隙，其中期望的结果是神经组织再生和功能恢复。到目前为止，将TENG转化为临床用于人类已经取得了一定程度的成功。为了优化TENG的设计并进一步接近TENG与自体神经移植物的匹配，许多新的线索，超越传统的，将不得不集成到TENG中。此外，对TENG建设相关的实时动态信息监控有着强烈的要求。这篇意见书的目的是具体和批判性地描述周围神经再生的神经组织工程领域的最新进展。在这里，我们描绘了新的尝试，在模板（或支架）材料的设计，特别是在生物相容性的背景下，选择和处理的支持细胞，和生长因子释放系统。我们进一步讨论了RNAi对周围神经再生的意义，预测了RNAi试剂在TENG中的潜在应用，并推测了其他元素的可能贡献，包括血管生成，电刺激，分子炎症介质，生物活性肽，抗氧化剂试剂和培养的生物构建体，TENG。最后，我们认为，必须在TENG内协调各种物理化学和生物学线索，以创建一个与周围神经系统的再生微环境非常接近的自洽协调系统。(C)2014爱思唯尔有限公司版权所有。
Tissue engineered nerve grafts (TENGs) have emerged as a potential alternative to autologous nerve grafts, the gold standard for peripheral nerve repair. Typically, TENGs are composed of a biomaterial-based template that incorporates biochemical cues. A number of TENGs have been used experimentally to bridge long peripheral nerve gaps in various animal models, where the desired outcome is nerve tissue regeneration and functional recovery. So far, the translation of TENGs to the clinic for use in humans has met with a certain degree of success. In order to optimize the TENG design and further approach the matching of TENGs with autologous nerve grafts, many new cues, beyond the traditional ones, will have to be integrated into TENGs. Furthermore, there is a strong requirement for monitoring the real-time dynamic information related to the construction of TENGs. The aim of this opinion paper is to specifically and critically describe the latest advances in the field of neural tissue engineering for peripheral nerve regeneration. Here we delineate new attempts in the design of template (or scaffold) materials, especially in the context of biocompatibility, the choice and handling of support cells, and growth factor release systems. We further discuss the significance of RNAi for peripheral nerve regeneration, anticipate the potential application of RNAi reagents for TENGs, and speculate on the possible contributions of additional elements, including angiogenesis, electrical stimulation, molecular inflammatory mediators, bioactive peptides, antioxidant reagents, and cultured biological constructs, to TENGs. Finally, we consider that a diverse array of physicochemical and biological cues must be orchestrated within a TENG to create a self-consistent coordinated system with a close proximity to the regenerative microenvironment of the peripheral nervous system. (C) 2014 Elsevier Ltd. All rights reserved.