Effects of dietary gamma-aminobutyric acid supplementation on the intestinal functions in weaning piglets
Effects of dietary gamma-aminobutyric acid supplementation on the intestinal functions in weaning piglets
复制标题
日粮中添加γ-氨基丁酸对断奶仔猪肠道功能的影响。
DOI：
10.1039/c8fo02161a
复制
发表时间：
2019-01-01
期刊：
影响因子：
6.1
通讯作者：
Yin, Yulong
中科院分区：
文献类型：
--
作者：
Chen, Shuai;Tan, Bie;Yin, Yulong
本研究旨在研究日粮中添加γ-氨基丁酸(GABA)对断奶仔猪生长性能、肠道免疫功能、肠道GABA能系统、氨基酸组成和肠道菌群的影响。将16头健康仔猪随机分为两组，分别饲喂基础日粮(CON组)和基础日粮中添加GABA(20 mg·kg~(-1))。每周监测体重和采食量。在添加GABA 3周后处死仔猪，采集血液、回肠、回肠粘膜和腔内容物。对免疫相关因子、GABA能系统、氨基酸谱、回肠微生物区系和血清氨基酸谱进行了探讨。结果表明，添加GABA可抑制IL-22、促炎性细胞因子(IL-1、IL-18)和MUC1的基因表达，促进抗炎细胞因子(干扰素-γ、IL-4和IL-10)、TLR6和MyD88的表达，从而改善生长性能，调节肠道免疫功能。GABA可调节肠道GABA能系统的少数成分，增加回肠粘膜中大部分氨基酸的水平，但降低血清氨基酸组成。GABA调节肠道微生物区系的数量和多样性，如优势微生物种群的丰富度、群落的丰富度和回肠微生物区系的多样性。综上所述，添加GABA可以调节肠道功能，包括肠道免疫功能、肠道氨基酸组成和肠道微生物区系，这一结果有助于了解GABA在肠道中的功能。
This study aims to investigate the effects of dietary gamma-aminobutyric acid (GABA) supplementation on the growth performance, intestinal immunity, intestinal GABAergic system, amino acid profiles and gut microflora of the weaned piglets. Totally sixteen healthy piglets were randomly assigned into two groups to be fed with the basal diet (Con group) or the basal diet with GABA (20 mg kg(-1)) supplementation. Body weights and feed intakes were monitored weekly. Piglets were sacrificed after 3 weeks of GABA supplementation to collect the blood, ileum, ileal mucosa and luminal content. Immune-associated factors, GABAergic system, amino acid profiles, and microbiota in the ileum and serum amino acid profiles were explored. The results showed that GABA supplementation improved the growth performance and modulated the intestinal immunity with inhibiting the gene expressions of IL-22, proinflammatory cytokines (IL-1 and IL-18), and Muc1, but promoted the expressions of anti-inflammatory cytokines (IFN-gamma, IL-4, and IL-10), TLR6 and MyD88. GABA regulated a few components of the intestinal GABAergic system, increased the levels of most amino acids in the ileal mucosa but reduced the serum amino acid profiles. GABA regulated the population and diversity of intestinal microbiota, such as the abundances of the dominant microbial populations, the community richness, and diversity of the ileal microbiota. In conclusion, GABA supplementation modulated the intestinal functions, including intestinal immunity, intestinal amino acid profiles and gut microbiota, and the results can be helpful for understanding the functions of GABA in the intestine.