Sphingosine-1-phosphate alleviates irradiation-induced parotid injury in a miniature pig model
Sphingosine-1-phosphate alleviates irradiation-induced parotid injury in a miniature pig model
复制标题
1-磷酸鞘氨醇减轻小型猪模型中辐射引起的腮腺损伤
DOI：
10.1111/odi.13302
复制
发表时间：
2020
期刊：
影响因子：
3.8
通讯作者：
Shan Zhaochen
中科院分区：
文献类型：
--
作者：
Pan Wen;Hu Liang;Chen Yawen;Zhu Zhao;Wang Yingxin;Song Jiaxin;Shan Zhaochen
目的我们的目的是在小型猪模型中验证1-磷酸鞘氨醇（S1P）的缓解效果。材料与方法在我们的实验中将30只雄性小型猪随机分为10组。我们在照射 (IR) 前 2 小时通过腮腺管以逆行方式施用 S1P。 IR 20 周后测试唾液流量和血流量。 IR后12、24小时和7天检查细胞凋亡水平。结果IR后20周，IR+S1P组IR侧腮腺唾液流量可维持在非IR侧的40%左右，而IR组仅维持20%。 IR + S1P 组的血流量和微血管密度显着高于 IR 组。 IR+S1P组细胞凋亡水平和cleaved caspase-3表达下调，Bcl-2/Bax比值升高。 IR 后 12、24 小时和 7 天，血流速率和 CD31 水平显着恢复。结论 1-磷酸鞘氨醇可能通过减少微血管内皮细胞凋亡和维持血流速率来部分缓解 IR 引起的腮腺功能障碍。
ObjectivesOur aim was to verify the alleviation effect of sphingosine‐1‐phosphate (S1P) in a miniature pig model.Material and methodsThirty male miniature pigs were randomly separated into 10 groups in our experiment. We administered S1P through the parotid duct in a retrograde fashion 2 hr before irradiation (IR). The salivary flow rate and blood flow rate were tested 20 weeks after IR. The apoptotic level was checked at 12, 24 hr and 7 days post‐IR.ResultsTwenty weeks after IR, the salivary flow rate of the IR‐side parotid gland in IR + S1P group can be maintained at about 40% of the non‐IR side, while only 20% was maintained in the IR group. The blood flow rate and microvascular density were significantly higher in the IR + S1P group than in the IR group. The apoptotic level and cleaved caspase‐3 expression were downregulated in IR + S1P group, and the ratio of Bcl‐2/Bax was increased. The blood flow rate and CD31 level were significantly restored at 12, 24 hr and 7 days post‐IR.ConclusionSphingosine‐1‐phosphate may partially alleviate IR‐induced parotid dysfunction by decreasing apoptosis of microvascular endothelial cells and maintaining the blood flow rate.