Conservation of Sox4 gene structure and expression during chicken embryogenesis
Conservation of Sox4 gene structure and expression during chicken embryogenesis
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DOI：
10.1016/j.gene.2003.07.002
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发表时间：
2003-11-27
期刊：
影响因子：
3.5
通讯作者：
Baldwin, HS
中科院分区：
文献类型：
--
作者：
Maschhoff, KL;Anziano, PQ;Baldwin, HS
Sox4是sry样HMG盒子基因家族的一员，其突变与多种人类疾病和小鼠胚胎缺陷有关，但Sox4在禽类胚胎中的结构和发育表达尚未被描述。我们分离并鉴定了鸡Sox4基因。鸡Sox4基因与小鼠和人类Sox4基因序列高度同源，特别是在hmg样DNA结合域和羧基端。此外，我们的原位杂交研究记录了胚胎发育期间的表达模式，与小鼠的表达模式非常相似，特别是在发育中的心脏表达方面。然而，在包括咽部弓和颅面中胚层在内的神经嵴起源组织中也检测到大量表达，支持先前在Sox4突变小鼠中检测到的神经嵴心脏病理的潜在主要作用。此外，与小鼠相比，在鸡发育中的神经管中检测到Sox4和Sox11的相互表达模式。这些研究表明，Sox4在鸡和小鼠这些结构的胚胎发育中起着重要且保守的作用，为进一步研究Sox4在器官发生关键事件中的作用奠定了基础。(C) 2003 Elsevier B.V.版权所有
While mutations in Sox4, a member of the SRY-like HMG box gene family, have been associated with a variety of human disorders and embryonic defects in the mouse, the structure and developmental expression of Sox4 in the avian embryo has not been described. We have isolated and characterized the chicken Sox4 gene. The chicken Sox4 gene shows a high degree of sequence homology with the mouse and human Sox4 genes, particularly in the HMG-like DNA binding domain and at the carboxy terminus. Furthermore, our in situ hybridization studies document an expression pattern during embryonic development that is very similar to that described for the mouse, particularly with regards to expression in the developing heart. However, abundant expression was also detected in tissues of neural crest origin including pharyngeal arch and craniofacial mesoderm, supporting a potential primary role in neural crest cardiac pathology previously detected in Sox4 mutant mice. Furthermore, a reciprocal pattern of Sox4 and Sox11 expression in the developing neural tube was detected in the chicken compared to that seen in the mouse. These studies suggest that Sox4 plays an important and conserved role in the embryonic development of these structures in the chicken as well as in the mouse and lay the foundation for future studies of the role of Sox4 during critical events of organogenesis. (C) 2003 Elsevier B.V. All rights reserved.