CHEMICAL STRUCTURES OF THE CORE REGION OF CAMPYLOBACTER-JEJUNI O/3 LIPOPOLYSACCHARIDE AND AN ASSOCIATED POLYSACCHARIDE
CHEMICAL STRUCTURES OF THE CORE REGION OF CAMPYLOBACTER-JEJUNI O/3 LIPOPOLYSACCHARIDE AND AN ASSOCIATED POLYSACCHARIDE
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DOI：
10.1111/j.1432-1033.1995.0570d.x
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发表时间：
1995-08-01
期刊：
影响因子：
--
通讯作者：
MORAN, AP
中科院分区：
文献类型：
--
作者：
ASPINALL, GO;LYNCH, CM;MORAN, AP
通过对苯酚/水萃取细菌细胞的空肠弯曲杆菌O:3型水不溶性低m (r)脂多糖衍生物的研究，确定了其核心低聚糖区域的完整结构。结构测定采用h -1核磁共振和p -31核磁共振波谱，甲基化分析支持快速原子轰击质谱，和史密斯降解实验。结论是，核心寡糖的完整链具有以下结构，其中一部分末端残基被磷酸化：[图]从类似的一系列实验中得出结论，从苯酚/水提取物的水相中分离出的相关多糖，具有以下重复单元，其中一部分先前未知的l-甘油- d -ido-庚糖（l- α - d -ido-hep）残基以3-羟基丙基酯的形式存在，并且不与脂多糖共价连接：-[-- bbbb3] - l- α - d -ido-hep -(1- bbbb4)- α - d - gal -(1-](n)-
The complete structure for the core oligosaccharide region of the water-insoluble low-M(r) lipopolysaccharide of Campylobacter jejuni serotype O:3 from phenol/water extraction of bacterial cells was assigned through studies on derivatives of the liberated oligosaccharide. Structure determinations were performed using H-1-NMR and P-31-NMR spectroscopies, methylation analysis supported by fast-atom-bombardment mass spectrometry, and Smith degradation experiments. It was concluded that the complete chains in the core oligosaccharide had the following structure in which a proportion of the terminal residues were phosphorylated:[GRAPHICS]From a similar series of experiments, it was concluded that an associated polysaccharide, which was isolated from the water phase of the phenol/water extracts, had the following repeating unit in which a proportion of the previously unknown L-glycero-D-ido-heptose (L-alpha-D-ido-Hep) residues were present as 3-hydroxypropanoyl esters, and were not covalently linked to the lipopolysaccharide:-[-->3)-L-alpha-D-ido-Hep-(1-->4)-alpha-D-Gal-(1-](n)-