ANALYSIS OF THIABENDAZOLE IN POTATOES AND APPLES BY ELISA USING MONOCLONAL-ANTIBODIES
ANALYSIS OF THIABENDAZOLE IN POTATOES AND APPLES BY ELISA USING MONOCLONAL-ANTIBODIES
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DOI：
10.1021/jf00030a032
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发表时间：
1993-06-01
影响因子：
6.1
通讯作者：
MONTAGUE, WC
中科院分区：
文献类型：
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作者：
BRANDON, DL;BINDER, RG;MONTAGUE, WC
2-(4-噻唑基)-1H-苯并咪唑[2-(4-噻唑)-1H-苯并咪唑，TBZ]被用作梨果类水果、柑橘、香蕉、土豆和其他几种商品的采前或采后杀菌剂。建立了一种用单抗与噻苯咪唑结合的酶联免疫吸附试验(ELISA)方法。抗体用半抗原5-琥珀酰胺基-2-(4-噻唑基)苯并咪唑激发。含水的缓冲液或水足以提取TBZ，而且该方法可以在4小时内完成，而不需要使用复杂的实验室设备。通过与使用层析分离和紫外检测的方法的比较，验证了结果的有效性。用水提苹果或土豆皮的酶联免疫吸附试验方法的检测限
Thiabendazole [2-(4-thiazolyl)-1H-benzimidazole,TBZ] is used as a preharvest or postharvest fungicide on pome fruits, citrus, bananas, potatoes, and several other commodities. An enzyme-linked immunosorbent (ELISA) method was developed which uses monoclonal antibodies that bind thiabendazole. The antibodies were elicited using the hapten 5-succinamido-2-(4-thiazolyl)benzimidazole. Aqueous buffers or water is adequate for extraction of the TBZ, and the method can be completed, without access to sophisticated laboratory equipment, within 4h. Results were validated by comparison to a method employing chromatographic fractionation with UV detection. The ELISA method using aqueous extraction of apple or potato peel has a limit of detection of