Phosphorylation of Nucleosides with Sodium cyclo-Triphosphate
Phosphorylation of Nucleosides with Sodium cyclo-Triphosphate
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DOI：
10.1246/bcsj.57.3274
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发表时间：
1984-11
影响因子：
4
通讯作者：
M. Tsuhako;Mayu Fujimoto;S. Ohashi;H. Nariai;I. Motooka
中科院分区：
文献类型：
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作者：
M. Tsuhako;Mayu Fujimoto;S. Ohashi;H. Nariai;I. Motooka
研究了环三磷酸钠（P3 m）对核苷的磷酸化反应。1)腺苷、胞苷、鸟苷和尿苷容易被P3 m磷酸化，选择性地形成2′-单磷酸核苷、3′-单磷酸核苷和2′，3 ′-环单磷酸核苷。另一方面，2′-脱氧腺苷、3′-脱氧腺苷、2′-脱氧胞苷和胸苷不能被P3 m磷酸化。2)腺苷、胞苷、鸟苷和尿苷的磷酸化强烈变化，这取决于反应条件;混合比、pH、反应温度和时间。在高配比（5：1-10：1）、高pH值（12-14）、高温（70-100 °C）短时间（1- 2 d）或低温（室温）长时间（70-150 d）条件下，可高产率（约50-100%）得到2′-和3′-单磷酸核苷。3)在腺苷酸磷酸化反应的初始阶段...
Phosphorylation of nucleosides with sodium cyclo-triphosphate (P3m) was studied under various conditions (mixing ratio of P3m to nucleoside, pH, reaction temperature and time). 1) Adenosine, cytidine, guanosine, and uridine were easily phosphorylated by P3m to form selectively nucleoside 2′-monophosphates, 3′-monophosphates, and 2′,3′-cyclic monophosphates. On the other hand, 2′-deoxyadenosine, 3′-deoxyadenosine, 2′-deoxycytidine, and thymidine could not be phosphorylated by P3m. 2) The phosphorylation of adenosine, cytidine, guanosine, and uridine varied strongly, depending on the reaction conditions; mixing ratio, pH, reaction temperature and time. Under conditions of a high mixing ratio of P3m to nucleoside (5:1–10:1), high pH (12–14), high temperature (70–100 °C) for a short time (1–2d) or low temperature (room temperature) for a long time (70–150 d), nucleoside 2′- and 3′-monophosphate could be obtained in high yield (about 50–100%). 3) At the initial stage of the phosphorylation reactions of adenosi...