Isolation and characterization of microsatellite loci in the endangered freshwater fish Varicorhinus alticorpus (Cyprinidae)
Isolation and characterization of microsatellite loci in the endangered freshwater fish Varicorhinus alticorpus (Cyprinidae)
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DOI：
10.1007/s10592-008-9508-2
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发表时间：
2008-01-01
影响因子：
2.2
通讯作者：
Lin, Hung-Du
中科院分区：
文献类型：
--
作者：
Chiang, Tzen-Yuh;Lee, Teh-Wang;Lin, Hung-Du
高角斑尾鱼（鲤科）是一种濒临灭绝的淡水鱼，分布在台湾南部和东部地区。我们描述了9个多态微卫星位点的发育，用于遗传研究。这些新的标记在20个稀有物种个体中进行了测试。等位基因数量在4 ~ 13个之间。期望杂合度（HE）和观察杂合度（H-O）分别为0.692 ~ 0.892（平均为0.821）和0.000 ~ 0.350（平均为0.088）。由于杂合子缺乏，所有位点都明显偏离Hardy-Weinberg预期。这组高度多态的微卫星提供了第一次在台湾进行该物种保护计划的机会。对其遗传多样性和精细群体结构进行评价是十分必要的。
Varicorhinus alticorpus (Cyprinidae) is ail endangered freshwater fish with a scattered distribution in the southern and eastern regions of Taiwan. We described the development of nine polymorphic microsatellite loci in V. alticorpus for genetic studies. These new markers were tested in 20 individuals of the rare species. The number of alleles ranged from 4 to 13. Expected (HE) and observed (H-O) heterozygosities ranged from 0.692 to 0.892 (averaged at 0.821) and from 0.000 to 0.350 (averaged at 0.088), respectively. All loci are significantly deviated from Hardy-Weinberg expectations due to the heterozygote deficiency. This suite of highly polymorphic microsatellites provides the first chance to undertake a conservation program for this species in Taiwan. It is essential to evaluate its genetic diversity and the finescale population structure.