Differential expression of caveolin-1 in lipopolysaccharide-activated murine macrophages
Differential expression of caveolin-1 in lipopolysaccharide-activated murine macrophages
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DOI：
10.1128/iai.68.9.5084-5089.2000
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发表时间：
2000-09-01
影响因子：
3.1
通讯作者：
Morrison, DC
中科院分区：
文献类型：
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作者：
Lei, MG;Morrison, DC
使用从具有正常脂多糖 (LPS) 反应性 (lps(n)) 的成纤维细胞和低反应性 (lps(d)) 成纤维细胞产生的 cDNA 进行五个相互消减杂交循环，发现 lps(d) 中的 Caveolin-1 mRNA 表达水平明显高于 lps(n) 成纤维细胞。 Caveolin-1 信息也可以通过逆转录 PCR 在 RAW264.7 和 J774.1 巨噬细胞样细胞系以及初级巯基乙酸 (TG) 诱导的小鼠腹膜巨噬细胞中轻松检测到。在 RAW264.7 细胞中，caveolin-1 mRNA 和蛋白质水平均被 LPS 下调。相反，在 TG 诱导的 C3HeB/FeJ 腹膜巨噬细胞中，caveolin-1 蛋白和 mRNA 的表达在体外响应 LPS 刺激而上调。在低至 1.0 pg LPS/ml 的浓度下，C3HeB/FeJ 腹膜巨噬细胞中的 Caveolin-1 蛋白表达上调。然而，需要高大约4个数量级的LPS浓度（10(4) pg/ml）来刺激LPS反应低下的C3H/HeJ小鼠腹膜巨噬细胞，从而检测到显着的caveolin-1蛋白上调。 Caveolin-1 是质膜小窝的主要成分，据报道是细胞刺激过程中信号转导的潜在重要调节因子。本报告中描述的结果表明，caveolin-1 表达可能与 LPS 信号传导/内化有关。
Five reciprocal cycles of subtractive hybridization using cDNA generated from fibroblasts with normal lipopolysaccharide (LPS) responsiveness (lps(n)) and from hyporesponsive (lps(d)) fibroblasts have led to the finding that caveolin-1 is expressed at markedly higher levels of mRNA in lps(d) than in lps(n) fibroblasts. Caveolin-1 message can also be readily detected via reverse transcription-PCR in the RAW264.7 and J774.1 macrophage-like cell lines as well as in primary thioglycolate (TG)-elicited mouse peritoneal macrophages. In RAW264.7 cells, both caveolin-1 mRNA and protein levels are down-regulated by LPS. In TG-elicited C3HeB/ FeJ peritoneal macrophages, in contrast, expression of both caveolin-1 protein and mRNA is up-regulated in vitro in response to LPS stimulation. The up-regulation of caveolin-1 protein expression in C3HeB/FeJ peritoneal macrophages tan be demonstrated at concentrations as low as 1.0 pg of LPS/ml. However, LPS concentrations approximately 4 orders of magnitude higher (10(4) pg/ml) were required to stimulate the LPS-hyporesponsive C3H/HeJ mice peritoneal macrophages such that significant caveolin-1 protein up-regulation was detected. Caveolin-1, a principal component of plasmalemmal caveolae, has been reported as a potentially important regulator for signal transduction during cellular stimulation. The results described in this report suggest that caveolin-1 expression may be associated with LPS signaling/internalization.