Sequence and expression of the gene for N10-formyltetrahydrofolate synthetase from Clostridium cylindrosporum.
Sequence and expression of the gene for N10-formyltetrahydrofolate synthetase from Clostridium cylindrosporum.
复制标题
柱孢梭菌 N10-甲酰四氢叶酸合成酶基因的序列和表达。
DOI：
10.1002/pro.5560020208
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Himes,RH
中科院分区：
文献类型：
--
作者：
Rankin,CA;Haslam,GC;Himes,RH
Sau3 A and Hind III restriction fragments ofClostridium cylindrosporumgenomic DNA were used to isolate clones containing 80% of theN10‐H4folate synthetase gene in a 5′ fragment and the remaining 20% of the gene in the 3′ fragment. These fragments were joined at a common SnaB I restriction site and expressed inEscherichia coliat a level equivalent to what is normally found inC. cylindrosporum.Sequence comparisons show a large degree of homology with genes from two other clostridial species, including a thermophile. Certain conserved sequences found in the three clostridial proteins and in theN10‐H4folate synthetase portion of eukaryotic Cl‐H4folate synthases may represent consensus sequences for nucleotide and H4folate binding.
