Polyvinylsulfonic acid: A Low-cost RNase inhibitor for enhanced RNA preservation and cell-free protein translation
Polyvinylsulfonic acid: A Low-cost RNase inhibitor for enhanced RNA preservation and cell-free protein translation
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DOI：
10.1080/21655979.2017.1313648
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发表时间：
2018-01-01
期刊：
影响因子：
4.9
通讯作者：
Bundy, Bradley C.
中科院分区：
文献类型：
--
作者：
Earl, Conner C.;Smith, Mark T.;Bundy, Bradley C.
报道了聚乙烯磺酸(PVSA)作为体外系统核糖核酸酶抑制剂的有效性和经济性。PVSA在核糖核酸酶A和大肠杆菌裂解物存在的情况下都能抑制RNA的切割，这表明PVSA可以作为一种更广泛的核糖核酸酶抑制物。此外，PVSA被证明可以将体外转录的mRNA的完整性提高5倍，这是以其翻译活性来衡量的。还报道了在普通RNA储存条件下，在PVSA存在的情况下，改善了mRNA转录本的保存。与商用核糖核酸酶抑制剂的成本比较表明，PVSA具有经济实用性，其价格约为常用核糖核酸酶抑制剂的1,700倍。PVSA也可以通过乙醇沉淀从RNA中分离出来，用于可能对PVSA存在敏感的应用。
The effectiveness and economics of polyvinyl sulfonic acid (PVSA) as a ribonuclease inhibitor for in vitro systems is reported. PVSA was shown to inhibit RNA cleavage in the presence of RNase A as well as in the presence of Escherichia coli lysate, suggesting that PVSA can act as a broader ribonuclease inhibitor. In addition, PVSA was shown to improve the integrity of mRNA transcripts by up to 5-fold in vitro as measured by their translational viability. Improved preservation of mRNA transcripts in the presence of PVSA under common RNA storage conditions is also reported. A cost comparison with commercially available RNAse inhibitors indicates the economic practicality of PVSA which is approximately 1,700times less expensive than commonly used ribonuclease inhibitors. PVSA can also be separated from RNA by alcohol precipitation for applications that may be sensitive to the presence of PVSA.