Differential expression of duplicated VAL-opsin genes in the developing zebrafish
Differential expression of duplicated VAL-opsin genes in the developing zebrafish
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DOI：
10.1111/j.1471-4159.2007.05093.x
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发表时间：
2008-03-01
影响因子：
4.7
通讯作者：
Dowling, John E.
中科院分区：
文献类型：
--
作者：
Kojima, Daisuke;Torii, Masaki;Dowling, John E.
Non-visual opsins mediate various light-dependent physiological events. Our previous search for non-visual opsin genes in zebrafish led to the discovery of VAL-opsin (VAL-opsinA) in deep brain cells and retinal horizontal cells of the adult fish. In this study, we report the identification and characterization of its duplicated gene, VAL-opsinB, in zebrafish. A molecular phylogenetic analysis indicates that VAL-opsinB is orthologous to a previously reported salmon gene and that the duplication of the VAL-opsin gene occurred in the teleost lineage. The recombinant protein of zebrafish VAL-opsinB forms a green-sensitive photopigment when reconstituted with 11-cis-retinal. VAL-opsinB expression was detected in a limited number of cells of the brain and the eye, and the expression pattern is distinct from that of the VAL-opsinA gene. Such a differential expression pattern suggests that VAL-opsinA and VAL-opsinB are involved in different physiological events in zebrafish.
