Sialic acid species as a determinant of the host range of influenza A viruses
Sialic acid species as a determinant of the host range of influenza A viruses
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DOI：
10.1128/jvi.74.24.11825-11831.2000
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发表时间：
2000-12-01
影响因子：
5.4
通讯作者：
Kawaoka, Y
中科院分区：
文献类型：
--
作者：
Suzuki, Y;Ito, T;Kawaoka, Y
唾液酸（SA）在动物物种中的分布是不同的，但这种变化的生物学作用在很大程度上是未知的。流感病毒识别sa -半乳糖（Gal）键的能力不同，这取决于它们是从哪种动物宿主分离出来的。例如，人类病毒优先识别通过alpha2,6（SA alpha2,6gal）连锁与Gal连接的SA，而马病毒则偏爱SA alpha2,3gal。然而，不同动物中特定SA物种（n -乙酰神经氨酸[NeuAc]和n -糖基神经氨酸[NeuGc]）的相对丰度差异是否会影响流感病毒的复制潜力尚不确定。因此，我们研究了对血凝素（HA）的需求，以支持病毒在马体内的复制，使用不同受体特异性的病毒。具有识别NeuAc alpha2,6gal但不识别NeuAc alpha2,3gal或NenGc alpha2,3gal的HA的病毒无法在马体内复制，而具有识别neuc alpha2,3gal片段的HA的病毒在马体内复制。此外，生化和免疫组织化学分析以及凝集素结合试验表明，马气管上皮细胞中NeuGca2,3Gal片段的丰度表明，对该片段的识别对马的病毒复制至关重要。因此，这些结果为不同SA物种在不同动物中的生物学效应提供了证据。
The distribution of sialic acid (SA) species varies among animal species, but the biological role of this variation is largely unknown. Influenza viruses differ in their ability to recognize SA-galactose (Gal) linkages, depending on the animal hosts from which they are isolated. For example, human viruses preferentially recognize SA linked to Gal by the alpha2,6(SA alpha2,6Gal) linkage, while equine viruses favor SA alpha2,3Gal. However, whether a difference in relative abundance of specific SA species (N-acetylneuraminic acid [NeuAc] and N-glycolylneuraminic acid [NeuGc]) among different animals affects the replicative potential of influenza viruses is uncertain. We therefore examined the requirement for the hemagglutinin (HA) for support of viral replication in horses, using viruses whose HAs differ in receptor specificity. A virus with an HA recognizing NeuAc alpha2,6Gal but not NeuAc alpha2,3Gal or NenGc alpha2,3Gal failed to replicate in horses, while one with an HA recognizing the NeuGc alpha2,3Gal moiety replicated in horses. Furthermore, biochemical and immunohistochemical analyses and a lectin-binding assay demonstrated the abundance of the NeuGca2,3Gal moiety in epithelial cells of horse trachea, indicating that recognition of this moiety is critical for viral replication in horses. Thus, these results provide evidence of a biological effect of different SA species in different animals.