Enrichment of Nitrifiers and the Influence of Antimicrobials on Nitrification during Batch Experiment
Enrichment of Nitrifiers and the Influence of Antimicrobials on Nitrification during Batch Experiment
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DOI：
10.2965/jwet.2015.37
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发表时间：
2015-01-01
影响因子：
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通讯作者：
Li, Yu-You
中科院分区：
文献类型：
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作者：
Du, Jingru;Tang, Chao;Li, Yu-You
实现了硝化细菌的富集，并计算了间歇实验期的污泥产量。采用批式试验研究了4种抗菌剂对氨氧化菌（AOB）和亚硝酸盐氧化菌（NOB）的影响。比氨氧化活性（SAA）为0.44 gN/gVSS/d，比亚硝酸盐氧化活性（SNA）为0.64 gN/gVSS/d。在400 mg/L以下，头孢氨苄（CLX）对硝化作用几乎没有不良影响，磺胺甲恶唑（SMX）对SAA和SNA的最高抑制率分别为15.4%和23.9%。阿奇霉素（AZI）的抑制作用最强。当AZI为400 mg/L时，AOB和NOB的相对活性分别为39.0%和29.8%。由于四环素（TC）与Ca 2+、Mg 2+的络合作用，生物量随着水面泡沫的出现而明显减少。在添加400 mg/L TC的条件下，当基质中含有钙离子、镁离子或两者同时存在时，污泥的剩余率分别为76.9%、13.7%和8.1%。
The enrichment of nitrifying bacteria was realized and sludge yield was calculated for the batch experiment period. The influence of four kinds of antimicrobials on ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) was investigated using batch experiments. The specific ammonia-oxidizing activity (SAA) was 0.44 gN/gVSS/d and the specific nitrite-oxidizing activity (SNA) was 0.64 gN/gVSS/d. Below 400 mg/L antimicrobials, Cefalexin (CLX) almost has no adverse effect on nitrification and the highest inhibition caused by Sulfamethoxazole (SMX) was 15.4% and 23.9% to SAA and SNA respectively. Azithromycin (AZI) exhibited the most intense inhibitory effect. The relative activity of AOB and NOB were 39.0% and 29.8% respectively when AZI was 400 mg/L. Due to the complexation of Tetracycline (TC) with Ca2+ and Mg2+, biomass showed obvious reduction with the foam on water surface. With the addition of 400 mg/L TC, only 76.9%, 13.7% and 8.1% of sludge remained when the substrate contained calcium or magnesium ions or both of them respectively.