A shotgun phosphoproteomics analysis of embryos in germinated maize seeds
A shotgun phosphoproteomics analysis of embryos in germinated maize seeds
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DOI：
10.1007/s00425-008-0805-2
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发表时间：
2008-11-01
期刊：
影响因子：
4.3
通讯作者：
Wang, Bai-Chen
中科院分区：
文献类型：
--
作者：
Lu, Tian-Cong;Meng, Ling-Bo;Wang, Bai-Chen
为了更好地了解可逆蛋白磷酸化在种子萌发中的作用，我们对萌发的玉米种子胚胎进行了磷酸化蛋白质组学研究。共鉴定出776个蛋白，包括39个激酶、16个磷酸酶和33个磷酸化蛋白，其中包含36个精确的体内磷酸化位点。除了HSP22上的磷酸化位点外，所有鉴定的磷酸化位点以前都没有报道过（Lund et al. in J Biol Chem, 276, 29924-29929, 2001）。QRT-PCR检测发现，10个激酶基因的转录本在种子萌发过程中显著上调，4个磷酸酶基因的转录本在种子萌发后显著上调，表明种子萌发过程中发生了可逆的蛋白磷酸化，激活了复杂的调控网络。这些磷酸化蛋白中至少有三分之一是与种子萌发相关的生物过程的关键成分，如DNA修复、基因转录、RNA剪接和蛋白质翻译，这表明蛋白质磷酸化在种子萌发中起着重要作用。据我们所知，这是首次对单子叶植物进行磷酸化蛋白质组学研究，为进一步研究种子萌发和幼苗发育的分子机制奠定了坚实的基础。
To better understand the role that reversible protein phosphorylation plays in seed germination, we initiated a phosphoproteomic investigation of embryos of germinated maize seeds. A total of 776 proteins including 39 kinases, 16 phosphatases, and 33 phosphoproteins containing 36 precise in vivo phosphorylation sites were identified. All the phosphorylation sites identified, with the exception of the phosphorylation site on HSP22, have not been reported previously (Lund et al. in J Biol Chem, 276, 29924-29929, 2001). Assayed with QRT-PCR, the transcripts of ten kinase genes were found to be dramatically up-regulated during seed germination and those of four phosphatase genes were up-regulated after germination, which indicated that reversible protein phosphorylation occurred and complex regulating networks were activated during this period. At least one-third of these phosphoproteins are key components involved in biological processes which relate to seed germination, such as DNA repair, gene transcription, RNA splicing and protein translation, suggesting that protein phosphorylation plays an important role in seed germination. As far as we know, this is the first phosphoproteomic study on a monocot and it will lay a solid foundation for further study of the molecular mechanisms of seed germination and seedling development.