Single-Cell Optogenetic Control of Calcium Signaling with a High-Density Micro-LED Array
Single-Cell Optogenetic Control of Calcium Signaling with a High-Density Micro-LED Array
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DOI：
10.1016/j.isci.2019.10.024
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发表时间：
2019-11-22
期刊：
影响因子：
5.8
通讯作者：
Xu, Guangyu
中科院分区：
文献类型：
--
作者：
Mao, Dacheng;Li, Ningwei;Xu, Guangyu
精确的光遗传学控制，理想情况下是致密细胞群中的单个细胞，对于理解细胞网络的异质性至关重要。具有这种能力的设备，如果以芯片规模构建，将在各种实验环境中在细胞水平上推进光遗传学研究。在这里，我们展示了在单细胞水平上使用16 μ m间距的微型发光二极管（LED）阵列，具有高亮度，小光斑尺寸，快速响应，和低电压操作的细胞内Ca2+动态的光遗传学控制。单个LED像素能够可靠地触发细胞内Ca2+瞬变，通过荧光显微镜和对照实验证实，并通过两种遗传编码的Ca2+指示剂进行交叉检查。重要的是，我们的阵列可以光遗传学地寻址在密集细胞群中相距小于10 μ m的单个细胞。这些结果表明，微LED阵列可能用于单细胞光遗传学的芯片实验室，这可能允许对各种细胞网络进行药物筛选和基础研究。
Precise optogenetic control, ideally down to single cells in dense cell populations, is essential in understanding the heterogeneity of cell networks. Devices with such capability, if built in a chip scale, will advance optogenetic studies at cellular levels in a variety of experimental settings. Here we demonstrate optogenetic control of intracellular Ca2+ dynamics at the single cell level using a 16-mu m pitched micro-light emitting diode (LED) array that features high brightness, small spot size, fast response, and low voltage operation. Individual LED pixels are able to reliably trigger intracellular Ca2+ transients, confirmed by fluorescence microscopy and control experiments and cross-checked by two genetically coded Ca2+ indicators, Importantly, our array can optogenetically address individual cells that are sub-10 mu m apart in densely packed cell populations. These results suggest the possible use of the micro-LED array toward a lab-on-a-chip for single-cell optogenetics, which may allow for pharmaceutical screening and fundamental studies on a variety of cell networks.