Effect of GRP75/mthsp70/PBP74/mortalin overexpression on intracellular ATP level, mitochondrial membrane potential and ROS accumulation following glucose deprivation in PC12 cells
Effect of GRP75/mthsp70/PBP74/mortalin overexpression on intracellular ATP level, mitochondrial membrane potential and ROS accumulation following glucose deprivation in PC12 cells
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DOI：
10.1007/s11010-005-2996-1
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发表时间：
2005-01-01
影响因子：
4.3
通讯作者：
Zuo, J
中科院分区：
文献类型：
--
作者：
Liu, Y;Liu, W;Zuo, J
葡萄糖调节蛋白75（glucose regulated protein 75，GRP75）是热休克蛋白（heat shock protein，HSP）家族中一种重要的分子伴侣。为了评估GRP75过表达对葡萄糖剥夺下的PC 12细胞的影响，在葡萄糖剥夺期间监测GRP75过表达的PC 12细胞和载体转染的对照PC 12细胞的细胞活力和线粒体功能。在暴露于葡萄糖剥夺后，GRP75过表达的PC 12细胞表现出比对照PC 12细胞更中度的细胞损伤。两组细胞在葡萄糖剥夺条件下均表现出ATP水平在早期增加后降低，并且两组细胞的线粒体电位也以类似的方式降低。对照PC 12细胞显示在GD处理开始后ROS积累的立即和快速增加，并且这种积累在GRP75过表达的PC 12细胞中减慢和减少。这些结果表明，GRP75可以抑制ROS的积累，这可能与GRP75过表达对葡萄糖剥夺的细胞保护作用有关。
Glucose regulated protein 75 (GRP75) is an important molecular chaperon belonged to the heat shock protein (HSP) family. To evaluate the effect of GRP75 overexpression on PC 12 cells under glucose deprivation, cell viability and mitochondrial function of GRP75-overexpressing PC 12 cells and the vector transfected control PC 12 cells were monitored during glucose deprivation. Upon exposure to glucose deprivation, GRP75-overexpressing PC 12 cells exhibited more moderate cell damage than control PC 12 cells. Both of the two groups of cells showed a decreased ATP level following an early increase in the condition of glucose deprivation, and the mitochondrial potential were also reduced in the similar manner in the two groups of cells. Control PC 12 cells showed an immediate and rapid increase in ROS accumulation after the onset of GD treatment, and this accumulation was slowed and reduced in GRP75-overexpressing PC12 cells. These findings suggested that GRP75 could inhibit the ROS accumulation, and it may be associated with the cytoprotective effect of GRP75 overexpression upon glucose deprivation.