Agronomic evaluation of tissue-culture-derived soybean plants
Agronomic evaluation of tissue-culture-derived soybean plants
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组织培养大豆植物的农艺评价
DOI：
10.1007/bf00226802
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发表时间：
1991
影响因子：
5.4
通讯作者：
J. Widholm
中科院分区：
文献类型：
--
作者：
P. A. Stephens;C. Nickell;J. Widholm
基于组织培养过程的再生植株的遗传改变（体细胞克隆变异）对于包括大豆[Glycine max（L.）Merr.]。本研究的目的是测试组织培养衍生的遗传变异的存在下，在8个农艺性状的纯合后代器官再生使用商业上重要的品种Asgrow 'A3127'。共86行来自9个再生植株的重复自花授粉生长在两个地点2年。与未再生亲本相比，成熟度、倒伏、株高、种子蛋白质和脂肪含量差异显著（P<0.05），但种子质量、种子重量和种子产量差异不显著。所有注意到的变化都是有益的，不涉及产量下降。由于差异不大，结果表明，组织培养过程不一定对植物性能有害，这是一个重要的考虑因素，因为组织培养技术用于许多基因工程方法。
SummaryGenetic alterations of regenerated plants based on the tissue culture process (somaclonal variation) have become common for many plant species including soybean [Glycine max (L.) Merr.]. The objective of this study was to test for the presence of tissue-culture-derived genetic variation in eight agronomic traits in homozygous progeny regenerated by organogenesis using the commercially important cultivar Asgrow ‘A3127.’ A total of 86 lines derived by repeated self-pollination of nine regenerated plants was grown in two locations for 2 years. When compared to the unregenerated parent, statistically significant variation (P<0.05) was found for maturity, lodging, height, seed protein and oil, but not for seed quality, seed weight, or seed yield. All of the variation noted was beneficial and did not involve decreased yield. Since the differences were not large, the results indicate that the tissue culture process is not necessarily detrimental to plant performance, which is an important consideration since tissue culture techniques are used in many genetic engineering methods.