Effect of Surface Modification of Silver Nanoparticles on the Proliferation of Human Lung Squamous Cell Carcinoma (HTB182) and Bronchial Epithelial (HBE) Cells In Vitro
Effect of Surface Modification of Silver Nanoparticles on the Proliferation of Human Lung Squamous Cell Carcinoma (HTB182) and Bronchial Epithelial (HBE) Cells In Vitro
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银纳米粒子表面修饰对人肺鳞状细胞癌（HTB182）和支气管上皮（HBE）细胞体外增殖的影响
DOI：
10.1166/jbn.2017.2419
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发表时间：
2017-10
影响因子：
2.9
通讯作者：
Liu Jian Xin
中科院分区：
文献类型：
--
作者：
Su Wei;Ma Liang;Wu Shang Hui;Li Wen;Tang Jian Xin;Deng Jing;Liu Jian Xin
通过AgNO 3水溶液与水溶性聚合物PEI和PEG反应，在体外形成稳定的银纳米颗粒（AgNPs）。TEM图像表明AgNP周围的可见层（10-35 nm）。使用细胞计数试剂盒-8（CCK-8）方法和光学显微镜评估2、4、8、10、20、30、40和50 μg/mL的AgNP对HTB 182肺癌细胞和正常人支气管上皮（HBE）细胞的毒性。结果表明，AgNPs对HTB 182和正常HBE细胞的毒性是剂量依赖性的，并且在癌细胞中更突出。流式细胞仪检测AgNPs和银离子对HTB 182和HBE细胞凋亡和细胞周期分布的影响。结果表明，AgNPs的表面修饰对其抗癌/抗增殖性能有很大影响。PEI-AgNPs对肿瘤细胞的抑制作用强于PEG-AgNPs。用AgNPs或银离子处理导致HBE细胞停滞在G 0/G1期。非小细胞肺癌HTB 182细胞由于DNA损伤而被阻滞于G2/M期。因此，银离子和AgNPs在正常HBE细胞中的作用机制不同于在肺癌细胞中的作用机制。
Stable silver nanoparticles (AgNPs) were formed in vitro by reacting AgNO3 (aq) solution with the water soluble polymers PEI and PEG. TEM images indicate a visible layer surrounding the AgNPs (10–35 nm). The toxicity of the AgNPs to HTB182 lung cancer cells and normal human bronchial epithelial (HBE) cells at 2, 4, 8, 10, 20, 30, 40 and 50 μg/mL was assessed using a Cell Counting Kit-8(CCK-8) method and optical microscopy. The results show that the toxicity of AgNPs to HTB182 and normal HBE cells was dose dependent and more prominent in the cancer cells. Then, the effect of AgNPs and silver ions on cell apoptosis and cell cycle distribution in HTB182 and HBE cells was determined by flow cytometry. The results showed that the surface modification of the AgNPs greatly affected their anti-cancer/anti-proliferative properties. The inhibitory effect of PEI-AgNPs on tumor cells was stronger than that of PEG-AgNPs. Treatment with AgNPs or silver ions led to HBE cell arrest in G0/G1. Non-small cell lung cancer HTB182 cells were arrested in G2/M phase due to DNA damage. Therefore, the mechanism of action of silver ions and AgNPs in normal HBE cells is different from that in lung cancer cells.