First Report on a Randomized Investigation of Antimicrobial Resistance in Fecal Indicator Bacteria from Livestock, Poultry, and Humans in Tanzania
First Report on a Randomized Investigation of Antimicrobial Resistance in Fecal Indicator Bacteria from Livestock, Poultry, and Humans in Tanzania
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DOI：
10.1089/mdr.2016.0297
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发表时间：
2018-04-01
影响因子：
2.6
通讯作者：
Olsen, John E.
中科院分区：
文献类型：
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作者：
Katakweba, Abdul A. S.;Muhairwa, Amandus P.;Olsen, John E.
本研究提供了坦桑尼亚人类（n=97）和食用动物（n=388）肠道指示细菌的抗菌素耐药性估计。超过70%的粪便样本含有四环素（TE），磺胺甲恶唑（STX），氨苄青霉素（AMP）耐药大肠菌群，而头孢噻肟（CTX）耐药大肠菌群中观察到40%的所有样本。来自人类的样本中CTX抗性大肠菌群的平均Log（10）菌落形成单位/g为2.20。390株大肠埃希菌对TE、STX、链霉素和CTX的耐药率分别为66.4%、54.9%、54.9%和36.4%。多重耐药率为65.1%。CTX耐药株均为bla（CTX-M）基因型。AMP和万古霉素耐药肠球菌很少见，阳性样本中的平均浓度较低（log（10）分别为0.9和0.4）。在240株肠球菌分离株中检测到低至中度耐药（2.1-15%），除利福平耐药（75.2%的分离株）外。sulII基因的平均拷贝数在Log（10）5.37和5.68之间变化，样品来源之间没有显著差异，而牛的tetW基因的数量显著高于人。基于随机获得的样本，这些发现将有助于为坦桑尼亚设计抗菌素耐药性（AMR）干预策略。
This study provides an estimate of antimicrobial resistance in intestinal indicator bacteria from humans (n=97) and food animals (n=388) in Tanzania. More than 70% of all fecal samples contained tetracycline (TE), sulfamethoxazole (STX), and ampicillin (AMP)-resistant coliforms, while cefotaxime (CTX)-resistant coliforms were observed in 40% of all samples. The average Log(10) colony forming units/g of CTX-resistant coliforms in samples from humans were 2.20. Of 390 Escherichia coli tested, 66.4% were resistant to TE, 54.9% to STX, 54.9% to streptomycin, and 36.4% to CTX. Isolates were commonly (65.1%) multiresistant. All CTX-resistant isolates contained bla(CTX-M) gene type. AMP- and vancomycin-resistant enterococci were rare, and the average concentrations in positive samples were low (log(10) 0.9 and 0.4, respectively). A low-to-moderate resistance (2.1-15%) was detected in 240 enterococci isolates to the drugs tested, except for rifampicin resistance (75.2% of isolates). The average number of sulII gene copies varied between Log(10) 5.37 and 5.68 with no significant difference between sample source, while cattle had significantly higher number of tetW genes than humans. These findings, based on randomly obtained samples, will be instrumental in designing antimicrobial resistance (AMR) intervention strategies for Tanzania.