A sensitive amperometric detection of neurotransmitter acetylcholine using carbon dot-modified carbon paste electrode
A sensitive amperometric detection of neurotransmitter acetylcholine using carbon dot-modified carbon paste electrode
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DOI：
10.1002/bab.1886
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发表时间：
2020-02-17
影响因子：
2.8
通讯作者：
Arslan, Fatma
中科院分区：
文献类型：
--
作者：
Bodur, Onur Can;Dinc, Saliha;Arslan, Fatma
乙酰胆碱是一种神经递质，位于内脏运动系统淋巴结和中枢神经系统各部分的神经和肌肉的交汇处。大脑中乙酰胆碱的减少与阿尔茨海默病有关。这就是为什么它是这种疾病的重要媒介。本研究采用纳米碳点-(3-氨丙基)三乙氧基硅烷(CDs-APTES)修饰的碳糊电极制备了具有乙酰胆碱酯酶和胆碱氧化酶的双酶生物传感器系统，用于测定乙酰胆碱的量。通过与戊二醛交联将乙酰胆碱酯酶和胆碱氧化酶固定到改性碳糊电极上。乙酰胆碱的测定是通过酶促产生的 H2O2 在 0.4 V 下相对于 Ag/AgCl 的氧化来进行的。研究了温度、pH 值和底物浓度对所制备的生物传感器的乙酰胆碱响应的影响。此外，还研究了最佳CDs-APTES量、生物传感器的线性工作范围和干扰效应。
Acetylcholine is a neurotransmitter, which is located at the intersections of the nerve and muscles in the lymph nodes of the internal organs motor systems and in various parts of the central nervous system. A decrease of acetylcholine in brain is associated with Alzheimer's disease. That is why it is an important agent for this disease. In this study, a bienzymatic biosensor system with acetylcholine esterase and choline oxidase was prepared with carbon paste electrode modified with carbon nano Dot-(3-Aminopropyl) triethoxysilane (CDs-APTES) for determination of the amount of acetylcholine. Acetylcholine esterase and choline oxidase enzymes were immobilized onto a modified carbon paste electrode by cross-linking with glutaraldehyde. Determination of acetylcholine was carried out by the oxidation of enzymatically produced H2O2 at 0.4 V versus Ag/AgCl. The effect of temperature, pH, and substrate concentration on the acetylcholine response of the prepared biosensor was investigated. In addition, the optimum CDs-APTES amount, the linear operating range of the biosensor, and the interference effect were also investigated.