In vitro infection of monocytes with HIVBa-L. Effect on cell surface expression of CD4, CD14, HLA-DR, and HLA-DQ.
In vitro infection of monocytes with HIVBa-L. Effect on cell surface expression of CD4, CD14, HLA-DR, and HLA-DQ.
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HIVBa-L 体外感染单核细胞。
DOI：
10.1089/aid.1990.6.731
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发表时间：
1990
影响因子：
1.5
通讯作者：
McDougal,JS
中科院分区：
文献类型：
--
作者：
Melendez-Guerrero,LM;Nicholson,JK;McDougal,JS
来自健康献血员的单核细胞在体外接种人类免疫缺陷病毒1型HIVBa-L的亲单核细胞株。在接种后第5天首次检测到HIV复制，在第17天达到病毒活性峰值。我们用流式细胞术检测了HIV感染和未感染的单核细胞/巨噬细胞（Mφ）上四种单核细胞表面抗原（CD 14、CD 4、HLA-DR和HLA-DQ）的表达动力学。我们一致地发现HIV感染的Mφ上的CD 4和CD 14的表达与未感染对照的Mφ上的CD 4和CD 14的表达相比降低。相反，HIV感染的Mφ上HLA-DR和HLA-DQ的表达没有变化。
Monocytes from healthy blood donors were inoculated in vitro with a monocyte-tropic strain of human immunodeficiency virus type 1 HIVBa-L. HIV replication was first detected at Day 5 postinoculation, with peak virus activity at Day 17. We assessed the kinetics of the expression of four monocyte surface antigens (CD14, CD4, HLA-DR, and HLA-DQ) on HIV-infected and uninfected monocyte/macrophages, (Mφ) by flow cytometry. We consistently found a decreased expression of CD4 and CD14 on HIV-infected Mφ compared to their expression on Mφ of uninfected controls. In contrast, HLA-DR and HLA-DQ expression was unchanged on HIV-infected Mφ.