EFFECT OF SODIUM VANADATE ON DEOXYRIBONUCLEIC-ACID AND PROTEIN SYNTHESES IN CULTURED RAT CALVARIAE
EFFECT OF SODIUM VANADATE ON DEOXYRIBONUCLEIC-ACID AND PROTEIN SYNTHESES IN CULTURED RAT CALVARIAE
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DOI：
10.1210/endo-116-3-855
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发表时间：
1985-01-01
期刊：
影响因子：
4.8
通讯作者：
CANALIS, E
中科院分区：
文献类型：
--
作者：
CANALIS, E
钒酸钠是一种已知具有多种细胞作用的药物，研究了其对21日龄胎鼠颅骨骨形成的影响。钒酸盐（0.1-10 .mu）M)刺激[3H]胸腺嘧啶并入酸不溶性残基（DNA）；作用时间为3 h，持续96 h。钒酸盐提高了骨DNA含量和有丝分裂指数。用钒酸盐处理10亩。M 24 h或0.3-1 .mu。M作用96 h可增加[3H]脯氨酸在胶原酶可消化蛋白（CDP）中的掺入，但对胶原无特异性影响；钒酸盐还增加了非胶原蛋白（NCP）的标记。钒酸盐增加了[3H]脯氨酸在I型胶原中的掺入，但不影响其他类型的胶原。钒酸盐（100亩）M)对DNA、CDP和NCP的标记产生明显的不可逆的抑制作用。多剂量钒酸盐处理3-96小时不会刺激碱性磷酸酶活性，但在暴露于1 mM钒酸盐24小时或10 .mu的骨骼中，该酶被抑制。对DNA标记的刺激作用主要在骨膜中观察到，而对CDP标记的刺激作用仅在无骨膜的骨中可见。显然，在体外，钒酸钠刺激骨DNA、胶原蛋白和NCP的合成，尽管高剂量的钒酸钠具有不可逆的抑制作用。
Sodium vanadate, an agent known to have multiple cellular actions, was studied for its effects on aspects of bone formation in cultures of 21 day old fetal rat calvariae. Vanadate (0.1-10 .mu.M) stimulated the incorporation of [3H]thymidine into acid-insoluble residues (DNA); the effect appeared after 3 h and was sustained for 96 h. Vanadate increased the bone DNA content and mitotic index. Treatment with vanadate at 10 .mu.M for 24 h or at 0.3-1 .mu.M for 96 h increased the incorporation of [3H]proline into collagenase-digestible protein (CDP), but the effect was not specific for collagen; vanadate also increased the labeling of noncollagen protein (NCP). Vanadate increased the incorporation of [3H]proline into type I collagen without affecting other collagen types. Vanadate (100 .mu.M) caused a marked and irreversible inhibitory effect on the labeling of DNA, CDP and NCP. Treatment with vanadate at multiple doses for 3-96 h did not stimulate alkaline phosphatase activity, but this enzyme was inhibited in bones exposed to 1 mM vanadate for 24 h or 10 .mu.M vanadate for 96 h. The stimulatory effect on DNA labeling was primarily observed in the periosteum, while that on CDP labeling was seen only in the periosteum-free bone. Apparently, sodium vanadate stimulates bone DNA, collagen and NCP syntheses in vitro, although high doses of vanadate have an irreversible inhibitory effect.