Map of differential transcript expression in the normal human large intestine
Map of differential transcript expression in the normal human large intestine
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DOI：
10.1152/physiolgenomics.00185.2006
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发表时间：
2008-03-14
影响因子：
4.6
通讯作者：
Young, Graeme P.
中科院分区：
文献类型：
--
作者：
LaPointe, Lawrence C.;Dunne, Robert;Young, Graeme P.
虽然有相当多的研究涉及到使用差异基因表达来预测疾病表型分类，例如。例如，在一个实施例中，肿瘤组织与非肿瘤对照相比，对正常组织中的表达范围了解甚少。了解非肿瘤组织中的基因表达模式，包括器官内的区域解剖表达变化，对于了解病变组织中的基因表达变化至关重要。为了探讨基因表达的变化沿着近端-远端轴的大肠，我们分析了微阵列数据在184个正常人标本使用单变量和多变量技术。我们发现219个探针组在近端和远端结直肠区域之间差异表达，115个探针组在末端片段之间差异表达，即，盲肠和直肠我们没有观察到任何两个相邻结直肠段之间存在统计学差异的探针组。占主导地位的表达模式（65探针集）遵循二分表达模式与肠的中肠-后肠胚胎起源一致，而第二种模式（50探针集）描绘了从盲肠到直肠的转录水平的逐渐变化。虽然二分模式包括大致相等数量的近端和远端升高的探针组，但几乎所有显示逐渐变化的探针组都表现出从近端到远端段的表达水平增加。这些模式描述了单个转录本变异的表达图谱以及沿大肠沿着的多基因表达模式。这是第一张完整人体器官的基因表达图谱。
While there is considerable research related to using differential gene expression to predict disease phenotype classification, e. g., neoplastic tissue from nonneoplastic controls, there is little understanding of the range of expression in normal tissues. Understanding patterns of gene expression in nonneoplastic tissue, including regional anatomic expression changes within an organ, is vital to understanding gene expression changes in diseased tissue. To explore the gene expression change along the proximal-distal axis of the large intestine, we analyzed microarray data in 184 normal human specimens using univariate and multivariate techniques. We found 219 probe sets that were differentially expressed between the proximal and distal colorectal regions and 115 probe sets that were differentially expressed between the terminal segments, i.e., the cecum and rectum. We did not observe any probe sets that were statistically different between any two contiguous colorectal segments. The dominant expression pattern (65 probe sets) follows a dichotomous expression pattern consistent with the midgut-hindgut embryonic origins of the gut while a second pattern (50 probe sets) depicts a gradual change in transcript levels from the cecum to the rectum. While the dichotomous pattern includes roughly equal numbers of probe sets that are elevated proximally and distally, nearly all probe sets that show a gradual change demonstrate increasing expression levels moving from proximal to distal segments. These patterns describe an expression map of individual transcript variation as well as multigene expression patterns along the large intestine. This is the first gene expression map of an entire human organ.