SURFACE OF LIVER CELL AFTER PARTIAL HEPATECTOMY
SURFACE OF LIVER CELL AFTER PARTIAL HEPATECTOMY
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DOI：
10.1016/0006-291x(65)90364-5
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发表时间：
1965-01-01
影响因子：
3.1
通讯作者：
LIEBERMAN.I
中科院分区：
文献类型：
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作者：
CHAUDHURI, S;LIEBERMAN.I
1960年首次报道了大鼠肝部分切除后肝细胞表面的变化，测量结果为电泳迁移率的增加。后来，在同一个实验室(2)和另一个实验室(3)又进行了进一步的研究，但没有研究表面变化的化学性质和病因。现在得到了与以下结论一致的证据。1)肝部分切除后肝细胞电泳率的增加是表面神经氨酸增加的结果；2)神经氨酸与可溶于30-95倍乙醇的分子结合；3)神经氨酸的出现取决于RNA和功能蛋白的合成。材料和方法选用体重约80g的本地雄性白化大鼠，神经氨酸酶(霍乱弧菌)、N-乙酰神经氨酸(NANA)、N-乙酰神经氨酸(N-Acetylmnnnosamine)来自通用生物化学公司，鼠李酸来自Sigma化学公司，放线菌素D来自Merck Sharp和Dohme研究实验室。部分肝切除术是指切除67%的肝脏(4)。假手术以相同的方式进行，只是没有切除肝脏。除非另有说明，否则肝脏样本是。在手术后15小时采集的假手术动物中，只有33%的部分
A change in the surface of the rat liver cell after partial hepatectomy, measured as an increase in electrophoretic mobility, was first reported in 1960 (1). Later, additional studies were made in the same (2) and another laboratory(3) but the chemical nature and the etiology of the surface alteration were not studied.Evidence consistent with the following conclusions has now been obtained. 1) The rise in electrophoretic mobility of the liver cell after partial hepatectomy results from an increase in surface neuraminate; 2) The neuraminate is bound to a molecule that is soluble in 30-95X ethanol; and 3) The appearance of the neuraminate is dependent upon the synthesis of RNA and functional protein. MATERIALS AND MEIRODS Male albino rats, obtained locally, were used when they weighed about 80 g. Neuraminidase (Vibrio cholerae), N-acetylneuraminic acid (NANA), and N-acetylmnnnosamine were from General Biochemicala, muramic acid was from the Sigma Chemical Company, and actinomycin D was a gift from Merck Sharp and Dohme Research Laboratories. Partial hepatectany refers to the removal of 67% of the liver (4). The sham operation was performed in the same way except that no liver was excised. Unless otherwise indicated, liver samples were. taken at 15 hours after the operations, and from sham-operated animals, only the 33% part