Comparative chlorophyll metabolic genes expression and response to dark stress of Ananas comosus var. bracteatus
Comparative chlorophyll metabolic genes expression and response to dark stress of Ananas comosus var. bracteatus
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DOI：
10.1080/14620316.2021.2023664
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发表时间：
2022-02
期刊：
影响因子：
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通讯作者：
Mark Owusu Adjei;Xuzixing Zhou;Yanbin Xue;Meiqin Mao;Huiling Zhang;Jiaheng Luo;Wei Yang;
中科院分区：
文献类型：
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作者：
Mark Owusu Adjei;Xuzixing Zhou;Yanbin Xue;Meiqin Mao;Huiling Zhang;Jiaheng Luo;Wei Yang;
叶绿素的积累是叶片着色和光合作用功能的基础。本研究分析了叶片再生白色和绿色（SLW和SLG）、组织培养白色和绿色（TCG）植株叶片叶绿素代谢机制的差异及其对30 d黑暗胁迫的响应。TCG和TCW的叶绿素含量分别高于SLG和SLW。叶绿素合成和循环基因，除非黄色着色1（NYC 1），表达明显高于绿色比白色叶部分。叶绿素酶（CLH）的明显上调和7-羟甲基叶绿素a还原酶（HCAR）的明显下调导致SLG中叶绿素含量低于TCG。CHLM和NOL表达下调可能是导致白化病SLW的原因之一。SGR、PAO和PPH的明显上调导致TCW变白。在30天的黑暗胁迫下，SLG和TCG叶片变白色。谷氨酰-tRNA还原酶1（HEMA 1）、原叶绿素氧化还原酶（POR）的下调和保持绿色（SGR）的上调可能导致SLG白化病。TCG的美白作用是由于CHLM和NOL的下调以及脱镁叶绿酸加氧酶（PAO）的上调所致。绿色叶色的丧失影响PGK和PsaA的表达，尤其是在TCG中。
ABSTRACT Chlorophyll accumulation is essential for leaf colouration and photosynthesis function. This study analysed chlorophyll metabolism mechanism differences between the Slip regenerated White (SLW) and Green (SLG) and Tissue Cultured White (TCW) and Green (TCG) plants’ leaf parts and their response to 30 d of dark stress. The TCG and TCW have higher chlorophyll content than the SLG and SLW, respectively. The chlorophyll biosynthesis and cycle genes, except Non-yellow colouring 1 (NYC1), are expressed apparently higher in green than in white leaf parts. The apparent up-regulation of Chlorophyllase (CLH) and down-regulation of 7-Hydroxymethyl Chlorophyll a reductase (HCAR) consequently caused the lower chlorophyll content in SLG compared to TCG. The down-regulation of CHLM and NOL may cause albino SLW. The apparent up-regulation of SGR, PAO, and PPH consequently caused the whitening of TCW. Under 30 days of dark stress, SLG and TCG leaves turned white. The down-regulation of Glutamyl-tRNA reductase 1 (HEMA1), Protochlorophyllide oxidoreductase (POR), and up-regulation of Stay Green (SGR) may result in SLG albino. The whitening of TCG is caused by the down-regulation of CHLM and NOL, also the up-regulation of Pheophorbide an oxygenase (PAO). The loss of green leaf colour affected PGK and PsaA expression, especially in TCG.