Cloning of a catalytic subunit of cAMP-dependent protein kinase from the honeybee (Apis mellifera) and its localization in the brain
Cloning of a catalytic subunit of cAMP-dependent protein kinase from the honeybee (Apis mellifera) and its localization in the brain
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DOI：
10.1046/j.1365-2583.2001.00252.x
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发表时间：
2001-04-01
影响因子：
2.6
通讯作者：
Menzel, R
中科院分区：
文献类型：
--
作者：
Eisenhardt, D;Fiala, A;Menzel, R
在蜜蜂中，cAMP依赖的信号转导级联与学习和记忆过程有关，cAMP依赖的蛋白激酶（PKA）是cAMP作用的主要介质。为了研究蜜蜂脑中PKA系统的结构，我们克隆了一个蜜蜂PKA催化亚基的同源物，推导的氨基酸序列与果蝇（Drosophilamelanogaster）、加州蜜蜂（Aaplasiacalifornica）和哺乳动物PKA催化亚基的同源性为80 - 94%。相应的基因主要在蘑菇体中表达，这是一种参与学习和记忆过程的结构。然而，也可以在触角和视叶中发现表达，在所有三个神经桩中表达水平不同。
In the honeybee the cAMP-dependent signal transduction cascade has been implicated in processes underlying learning and memory, The cAMP-dependent protein kinase (PKA) is the major mediator of cAMP action. To characterize the PKA system in the honeybee brain we cloned a homologue of a PKA catalytic subunit from the honeybee,The deduced amino acid sequence shows 80-94% identity with catalytic subunits of PKA from Drosophila melanogaster, Aplysia californica and mammals. The corresponding gene is predominantly expressed in the mushroom bodies, a structure that is involved in learning and memory processes. However, expression can also be found in the antennal and optic lobes,The level of expression varies within all three neuropiles.