Pinus canariensis plant regeneration through somatic embryogenesis
Pinus canariensis plant regeneration through somatic embryogenesis
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DOI：
10.5424/fs/2020291-16136
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发表时间：
2020-01-01
期刊：
影响因子：
0.7
通讯作者：
Itziar, Montalban A.
中科院分区：
文献类型：
--
作者：
Ander, Castander-Olarieta;Paloma, Moncalean;Itziar, Montalban A.
研究目的：为了开发一种有效的方法来再生植物，通过体细胞胚胎发生的生态相关的树种，如Pinuscanariensis.Area的研究：这项研究是在研究实验室的Neiker-Tecnalia（Arkaute，西班牙）。材料和方法：绿色圆锥体的两个收集日期的Pinuscanariensis进行处理，并产生的未成熟合子胚培养在三个基本培养基。对启动的胚性组织进行增殖，测试两种继代频率，并对获得的胚性细胞系进行成熟。对所产生的体细胞胚进行了萌发，并在温室中进行了驯化。主要结果：获得了增殖活跃的胚性细胞系，并将成功萌发的发育良好的体细胞胚驯化在温室中，显示出适当的生长。这是第一份关于加拿大松体细胞胚胎发生的报告，为这一物种的研究目的和大规模无性繁殖的强大生物技术工具开辟了道路。
Aim of the study: To develop an efficient method to regenerate plants through somatic embryogenesis of an ecologically relevant tree species such as Pinus canariensis.Area of study: The study was conducted in the research laboratories of Neiker-Tecnalia (Arkaute, Spain).Material and methods: Green cones of Pinus canariensis from two collection dates were processed and the resulting immature zygotic embryos were cultured on three basal media. The initiated embryogenic tissues were proliferated testing two subculture frequencies, and the obtained embryogenic cell lines were subjected to maturation. Germination of the produced somatic embryos was conducted and acclimatization was carried out in a greenhouse under controlled conditions.Main results: Actively proliferating embryogenic cell lines were obtained and well-formed somatic embryos that successfully germinated were acclimatized in the greenhouse showing a proper growth.Research highlights: This is the first report on Pinus canariensis somatic embryogenesis, opening the way for a powerful biotechnological tool for both research purposes and massive vegetative propagation of this species.