Cartilage on the move: cartilage lineage tracing during tadpole metamorphosis.
Cartilage on the move: cartilage lineage tracing during tadpole metamorphosis.
复制标题
DOI：
10.1111/dgd.12002
复制
发表时间：
2012-10
期刊：
影响因子：
--
通讯作者：
Buchholz DR
中科院分区：
文献类型：
--
作者：
Kerney RR;Brittain AL;Hall BK;Buchholz DR
蝌蚪变态期颅软骨的重组是一系列复杂的过程。幼虫软骨形成细胞（软骨细胞）的命运和成人软骨细胞的来源在很大程度上是未知的。个别幼虫颅软骨可能会退化或重塑，而许多成人软骨出现在变态过程中从头形成。确定在何种程度上成人软骨细胞/软骨来自幼虫软骨细胞在变态过程中，需要软骨细胞谱系追踪的新技术。我们已经开发了两种转基因系统，用荧光蛋白标记全身的软骨细胞。一种系统仅强烈标记早期蝌蚪软骨。另一个系统在任何发育阶段诱导标记形成的软骨。我们检查了头骨（内脏和脑颅）的软骨，并确定幼虫软骨吸收或重塑成成人软骨。我们的数据表明，成人的耳囊，顶盖前和后，透明，和部分Meckel的软骨来自幼虫的软骨细胞。我们的数据还表明，大多数成人软骨从头形成，虽然我们不能排除极端的幼虫软骨细胞增殖或去分化和再分化的可能性，这可能会稀释我们的荧光蛋白信号。这里开发的转基因谱系追踪策略是非洲爪蟾中诱导的、特异性的、谱系追踪的第一个例子。
The reorganization of cranial cartilages during tadpole metamorphosis is a set of complex processes. The fates of larval cartilage-forming cells (chondrocytes) and sources of adult chondrocytes are largely unknown. Individual larval cranial cartilages may either degenerate or remodel, while many adult cartilages appear to form de novo during metamorphosis. Determining the extent to which adult chondrocytes/cartilages are derived from larval chondrocytes during metamorphosis requires new techniques in chondrocyte lineage tracing. We have developed two transgenic systems to label cartilage cells throughout the body with fluorescent proteins. One system strongly labels early tadpole cartilages only. The other system inducibly labels forming cartilages at any developmental stage. We examined cartilages of the skull (viscero- and neurocranium), and identified larval cartilages that either resorb or remodel into adult cartilages. Our data show that the adult otic capsules, tecti anterius and posterius, hyale, and portions of Meckel’s cartilage are derived from larval chondrocytes. Our data also suggest that most adult cartilages form de novo, though we cannot rule out the potential for extreme larval chondrocyte proliferation or de- and re-differentiation, which could dilute our fluorescent protein signal. The transgenic lineage tracing strategies developed here are the first examples of inducible, skeleton-specific, lineage tracing in Xenopus.
登录
查看更多内容
影响因子：
2.5
作者：
Rankin, Scott A.;Zorn, Aaron M.;Buchholz, Daniel R.
通讯作者：
Buchholz, Daniel R.
影响因子：
4.6
作者：
Abzhanov, Arhat;Rodda, Stephen J.;Tabin, Clifford J.
通讯作者：
Tabin, Clifford J.
影响因子：
2.6
作者：
Gross, Joshua B.;Hanken, James
通讯作者：
Hanken, James
影响因子：
2.5
作者：
Kerney, Ryan;Gross, Joshua B.;Hanken, James
通讯作者：
Hanken, James
DOI：
10.1073/pnas.0803569105
发表时间：
2008-07-01
影响因子：
11.1
作者：
Mukhi, Sandeep;Mao, Jinzhe;Brown, Donald D.
通讯作者：
Brown, Donald D.