Effect of low-level laser therapy (GaAs 904 nm) in skeletal muscle fatigue and biochemical markers of muscle damage in rats
Effect of low-level laser therapy (GaAs 904 nm) in skeletal muscle fatigue and biochemical markers of muscle damage in rats
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DOI：
10.1007/s00421-009-1321-1
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发表时间：
2010-04-01
影响因子：
3
通讯作者：
Bjordal, Jan Magnus
中科院分区：
文献类型：
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作者：
Pinto Leal Junior, Ernesto Cesar;Brandao Lopes-Martins, Rodrigo Alvaro;Bjordal, Jan Magnus
我们想要测试904 nm激光运动前肌肉照射是否影响强直性收缩大鼠模型的疲劳、血乳酸水平和肌酸激酶(CK)活性。30只雄性Wistar大鼠随机分为5组，分别接受4种不同剂量(0.1、0.3、1.0和3.0J)激光照射或不治疗对照组。治疗组在第一次收缩前立即进行激光照射。电刺激诱发6次强直性胫前肌收缩，每次收缩间隔10min。当肌肉力量下降到每次收缩峰值的50%时，收缩停止；在第一次收缩之前和第六次收缩后立即采集血液样本。6次收缩的相对峰力在最高剂量的两个激光照射组[1.0J为151.27%(SD+/-A18.82)，3.0J为144.84%(SD+/-A34.47)，对照组为82.25%(SD+/-A11.69)]明显优于对照组[SD+/-A18.82%(SD+/-A18.82)]。1.0和3.0J组在第六次收缩中的平均作功也有类似的显著增加(P<；0.05)。所有照射组的血乳酸水平均显著低于对照组(P<；0.05)。除3.0J组外，其余各照射组运动后CK活性均显著低于对照组。结论：1.0J、904 nm波长的激光运动前照射可显著延缓大鼠运动后肌肉疲劳，降低运动后血乳酸和CK水平。
We wanted to test if pre-exercise muscle irradiation with 904 nm laser affects the development of fatigue, blood lactate levels and creatine kinase (CK) activity in a rat model with tetanic contractions. Thirty male Wistar rats were divided into five groups receiving either one of four different laser doses (0.1, 0.3, 1.0 and 3.0 J) or a no-treatment control group. Laser irradiation was performed immediately before the first contraction for treated groups. Electrical stimulation was used to induce six tetanic tibial anterior muscle contractions with 10 min intervals between them. Contractions were stopped when the muscle force fell to 50% of the peak value for each contraction; blood samples were taken before the first and immediately after the sixth contraction. The relative peak forces for the sixth contraction were significantly better (P < 0.05) in the two laser groups irradiated with highest doses [151.27% (SD +/- A 18.82) for 1.0 J, 144.84% (SD +/- A 34.47) for 3.0 J and 82.25% (SD +/- A 11.69) for the control group]. Similar significant (P < 0.05) increases in mean performed work during the sixth contraction for the 1.0 and 3.0 J groups were also observed. Blood lactate levels were significantly lower (P < 0.05) than the control group in all irradiated groups. All irradiated groups except the 3.0 J group had significantly lower post-exercise CK activity than the control group. We conclude that pre-exercise irradiation with a laser dose of 1.0 J and 904 nm wavelength significantly delays muscle fatigue and decreases post-exercise blood lactate and CK in this rat model.