Layer by layer three-dimensional tissue epitaxy by cell-laden hydrogel droplets.
Layer by layer three-dimensional tissue epitaxy by cell-laden hydrogel droplets.
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DOI：
10.1089/ten.tec.2009.0179
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发表时间：
2010-02
期刊：
影响因子：
--
通讯作者：
Demirci U
中科院分区：
文献类型：
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作者：
Moon S;Hasan SK;Song YS;Xu F;Keles HO;Manzur F;Mikkilineni S;Hong JW;Nagatomi J;Haeggstrom E;Khademhosseini A;Demirci U
生物工程三维(3D)组织的能力是治疗各种疾病(如癌症、组织功能丧失或器官衰竭)的潜在有效方法。然而，传统的组织工程方法在制造类似天然组织微血管和微结构的3D组织结构方面面临挑战。我们已经开发了一种生物打印机，可以用于打印包裹在胶原中的3D平滑肌细胞斑块(5 mm×5 mm×81μm)。当前的喷墨打印系统存在细胞活性丧失和堵塞的问题。为了克服这些限制，我们开发了一种使用机械阀门打印包含细胞的高粘度水凝胶前体的系统。我们开发的生物打印平台能够(I)以受控的空间分辨率(近轴：18.0±7.0μm，远轴：0.5±4.9μm)打印多层3D载细胞水凝胶结构(每层16.2μm厚)，(Ii)高通量产生液滴(每层1个S，160个液滴/S)，(Iii)细胞种植均匀度(100万个细胞/毫升时为26±2个细胞/毫米~2,500万个细胞/毫升时为122±20个细胞/毫米~2，千万个细胞/毫升时为216±38个细胞/毫米~2)，以及(Iv)在培养中的长期生存能力(>；90%，14天)。通过能够制造打印的替代组织，该打印3D组织构建物的平台可能有益于再生医学应用。
The ability to bioengineer three-dimensional (3D) tissues is a potentially powerful approach to treat diverse diseases such as cancer, loss of tissue function, or organ failure. Traditional tissue engineering methods, however, face challenges in fabricating 3D tissue constructs that resemble the native tissue microvasculature and microarchitectures. We have developed a bioprinter that can be used to print 3D patches of smooth muscle cells (5 mm×5mm×81 μm) encapsulated within collagen. Current inkjet printing systems suffer from loss of cell viability and clogging. To overcome these limitations, we developed a system that uses mechanical valves to print high viscosity hydrogel precursors containing cells. The bioprinting platform that we developed enables (i) printing of multilayered 3D cell-laden hydrogel structures (16.2 μm thick per layer) with controlled spatial resolution (proximal axis: 18.0 ± 7.0 μm and distal axis: 0.5±4.9 μm), (ii) high-throughput droplet generation (1 s per layer, 160 droplets/s), (iii) cell seeding uniformity (26±2 cells/mm2 at 1 million cells/mL, 122±20 cells/mm2 at 5 million cells/mL, and 216±38 cells/mm2 at 10 million cells/mL), and (iv) long-term viability in culture (>90%, 14 days). This platform to print 3D tissue constructs may be beneficial for regenerative medicine applications by enabling the fabrication of printed replacement tissues.